Rodrigues Carmem R F, Dias Jacqueline H, Semedo Juliane G, da Silva Juliana, Ferraz Alexandre B F, Picada Jaqueline N
Programa de Pós-Graduação em Genética e Toxicologia Aplicada, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil.
J Ethnopharmacol. 2009 Jul 15;124(2):321-4. doi: 10.1016/j.jep.2009.04.022. Epub 2009 May 3.
Baccharis dracunculifolia (D.C.) (Asteraceae), a native plant to Brazil known as "vassourinha" or "alecrim-do-campo", is the most important botanical source of a Brazilian propolis called green propolis. The leaf extracts of this plant have been used to treat liver and digestive disorders. It has been recognized that green propolis can induce mutagenic effects at high doses, but no study reporting possible mutagenic effects by Baccharis dracunculifolia extracts in the maximum tolerated dose has been conducted. The aim of the present study was to investigate the genotoxic and mutagenic effects of this plant in vivo. Adult CF-1 mice were treated with 0.5g/kg, 1.0g/kg or 2.0g/kg of an aqueous extract of Baccharis dracunculifolia by gavage for 3 consecutive days. Blood and liver samples were collected to detect DNA damage using the comet assay, while bone marrow samples were used to assess chromosome mutations by the micronucleus test. The extract increased the DNA damage in blood and liver tissues and the frequency of micronucleus in bone marrow. These findings suggest genotoxic and mutagenic effects of Baccharis dracunculifolia comparable to green propolis in mice.
巴西酒神菊（Baccharis dracunculifolia (D.C.)，菊科）是一种原产于巴西的植物，被称为“小扫帚”或“田野百里香”，是一种名为绿蜂胶的巴西蜂胶的最重要植物来源。这种植物的叶提取物已被用于治疗肝脏和消化系统疾病。人们已经认识到，高剂量的绿蜂胶会诱发诱变效应，但尚未有研究报告巴西酒神菊提取物在最大耐受剂量下可能产生的诱变效应。本研究的目的是在体内研究这种植物的遗传毒性和诱变效应。成年CF-1小鼠连续3天经口灌胃给予0.5g/kg、1.0g/kg或2.0g/kg的巴西酒神菊水提取物。采集血液和肝脏样本，使用彗星试验检测DNA损伤，同时使用骨髓样本通过微核试验评估染色体突变。提取物增加了血液和肝脏组织中的DNA损伤以及骨髓中的微核频率。这些发现表明，巴西酒神菊在小鼠体内具有与绿蜂胶相当的遗传毒性和诱变效应。
