Brindo da Cruz Isabele Camargo, Velosa Ana Paula Pereira, Carrasco Solange, Dos Santos Filho Antonio, Tomaz de Miranda Jurandir, Pompeu Eduardo, Fernandes Tiago Lazzaretti, Bueno Daniela Franco, Fanelli Camila, Goldenstein-Schainberg Cláudia, Fabro Alexandre Todorovic, Fuller Ricardo, Silva Pedro Leme, Capelozzi Vera Luiza, Teodoro Walcy Rosolia
Rheumatology Division of the Hospital das Clinicas, Faculdade de Medicina, Universidade de São Paulo, FMUSP, São Paulo, Brazil.
Bioterism Center of the Hospital das Clinicas, Faculdade de Medicina, Universidade de São Paulo, FMUSP, São Paulo, Brazil.
Front Cell Dev Biol. 2021 Mar 22;9:606890. doi: 10.3389/fcell.2021.606890. eCollection 2021.
Collagen is essential for cartilage adhesion and formation. In the present study, histology, immunofluorescence, morphometry, and qRT-PCR suggested that adipose-derived stem cells (ADSCs) stimulated by type V collagen (Col V) induce a significant increase of type II collagen (Col II) in the degenerative area of surgical-induced osteoarthritic rabbit articular cartilage (OA). , the effects of Col V on the proliferation and differentiation of ADSC were investigated. The expression of the cartilage-related genes and was significantly upregulated and was downregulated post-ADSC/Col V treatment. Post-ADSC/Col V treatment, analyses revealed that rabbits showed typical signs of osteoarthritic articular cartilage regeneration by hematoxylin and eosin (H&E) and Safranin O/Fast Green staining. Immunohistochemical staining demonstrated that the volume of Col II fibers and the expression of Col II protein were significantly increased, and apoptosis Fas ligand positive significantly decreased post-ADSC/Col V treatment. In conclusion, the expression of Col II was higher in rabbits with surgical-induced osteoarthritic articular cartilage; hence, ADSC/Col V may be a promising therapeutic target for OA treatment.
胶原蛋白对于软骨的黏附和形成至关重要。在本研究中，组织学、免疫荧光、形态计量学和定量逆转录聚合酶链反应表明，由V型胶原蛋白（Col V）刺激的脂肪来源干细胞（ADSCs）可使手术诱导的骨关节炎兔关节软骨（OA）退变区域的II型胶原蛋白（Col II）显著增加。 ，研究了Col V对ADSC增殖和分化的影响。ADSC/Col V处理后，软骨相关基因 和 的表达显著上调，而 下调。ADSC/Col V处理后， 分析显示，通过苏木精和伊红（H&E）以及番红O/固绿染色，兔子表现出骨关节炎关节软骨再生的典型迹象。免疫组织化学染色表明，ADSC/Col V处理后，Col II纤维体积和Col II蛋白表达显著增加，凋亡Fas配体阳性显著降低。总之，手术诱导的骨关节炎关节软骨兔中Col II的表达较高；因此，ADSC/Col V可能是OA治疗的一个有前景的治疗靶点。
