Xiangya Hospital of Central South University, Changsha 410008, PR China.
Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, PR China.
Fitoterapia. 2018 Oct;130:203-209. doi: 10.1016/j.fitote.2018.09.005. Epub 2018 Sep 11.
Four new trace alkaloids with lycodine-related structures, Lycocasuarinines A-D (1-4), together with seven known analogues (5-11), were isolated from the chloroform extract of Lycopodiastrum casuarinoides. The structures and stereochemistry of 1-4 were elucidated by spectroscopic analysis (IR, UV, MS, NMR, HRESIMS and CD) and comparison with known ones. The acetylcholinesterase (AChE) and butyrocholinesterase (BuChE) inhibitory activities of nine isolates were evaluated. Lycocasuarinine D (4) showed the most potent AChE inhibitory effect. In addition, a plausible biogenetic pathway of compound 4 was proposed.
Lycocasuarinines A-D（1-4），以及七个已知类似物（5-11）。通过光谱分析（IR、UV、MS、NMR、HRESIMS 和 CD）以及与已知化合物的比较，确定了 1-4 的结构和立体化学。对 9 种分离物的乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）抑制活性进行了评价。Lycocasuarinine D（4）对 AChE 具有最强的抑制作用。此外，还提出了化合物 4 的可能生物合成途径。
