[具体物种]及其同属分类群的微卫星标记的特征分析与开发
Characterization and development of microsatellite markers for and congeneric taxa.
作者信息
Zumwalde Bethany A, Dahir Josie K, Shaw Allyson B, Willis Alison, Fehlberg Shannon D
机构信息
Department of Research, Conservations and Collections Desert Botanical Garden 1201 N. Galvin Parkway Phoenix Arizona 85008 USA.
出版信息
Appl Plant Sci. 2019 Nov 8;7(11):e11302. doi: 10.1002/aps3.11302. eCollection 2019 Nov.
PREMISE
Microsatellite markers were developed in (Cactaceae) for use in several morphologically similar, closely related taxa within the genus to study genetic structure and diversity within and among individuals and populations.
METHODS AND RESULTS
Using reads from shallow, whole genome Illumina HiSeq high-throughput sequencing, we developed and characterized 15 microsatellite primer pairs for , var. , var. , and . Of the 15 microsatellite markers, 14 amplified successfully and were polymorphic in three of the four taxa tested, with the exception of three markers in . In , the number of alleles ranged from one to 15 and levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and 0.000 to 0.917, respectively.
CONCLUSIONS
These markers will be useful for investigating population genetics and clarifying taxonomic relationships of and congeneric species.
前提
在仙人掌科中开发了微卫星标记，用于该属内几个形态相似、亲缘关系密切的分类群，以研究个体和种群内部以及之间的遗传结构和多样性。
方法与结果
利用来自浅层全基因组Illumina HiSeq高通量测序的读数，我们为[具体物种]、[变种1]、[变种2]和[变种3]开发并鉴定了15对微卫星引物对。在这15个微卫星标记中，14个成功扩增，并且在四个测试分类群中的三个中具有多态性，但[具体物种]中有三个标记除外。在[具体物种]中，等位基因数量从1到15不等，观察到的杂合度和预期杂合度水平分别从0.000到1.000和0.000到0.917。
结论
这些标记将有助于研究[具体物种]及其同属物种的群体遗传学并阐明分类关系。
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