Scheurlen W, Stremlau A, Gissmann L, Höhn D, Zenner H P, zur Hausen H
Int J Cancer. 1986 Nov 15;38(5):671-6. doi: 10.1002/ijc.2910380509.
By hybridization under stringent conditions, one out of two anal carcinomas and one out of 36 laryngeal carcinomas were shown to harbor HPV 16 DNA in high copy number. Further analysis of both tumor DNAs indicated a rearrangement of the viral DNA in the tumor cells. HPV 16 DNA in the anal carcinoma could chiefly be found episomally in two different forms: a minority as 7.9-kb oligomeric episomes with no apparent modifications; as 10.7-kb rear-ranged oligomeric episomes with a duplication of the part of the viral genome encoding the open reading frames (ORF) E7, E1 and parts of E6 and E2. In the laryngeal carcinoma, integrated and episomal HPV 16 DNA molecules of 7.9 kb were present, together with rearranged molecules of approximately 18 kb with multiple duplications of the ORF E4 and parts of the ORFs E2, E5, L1 and L2. Possible consequences for transcription of the modified viral genomes are discussed.
通过在严格条件下进行杂交，发现两例肛管癌中有一例、36例喉癌中有一例含有高拷贝数的人乳头瘤病毒16型（HPV 16）DNA。对这两种肿瘤DNA的进一步分析表明，肿瘤细胞中的病毒DNA发生了重排。肛管癌中的HPV 16 DNA主要以两种不同形式游离存在：少数为7.9 kb的寡聚游离型，无明显修饰；多数为10.7 kb的重排寡聚游离型，病毒基因组中编码开放阅读框（ORF）E7、E1以及部分E6和E2的区域发生了重复。在喉癌中，存在7.9 kb的整合型和游离型HPV 16 DNA分子，以及约18 kb的重排分子，其中ORF E4以及部分ORF E2、E5、L1和L2发生了多次重复。文中讨论了修饰后的病毒基因组转录可能产生的后果。
