Zarafu Irina, Turcu Ioana, Culiță Daniela C, Petrescu Simona, Popa Marcela, Chifiriuc Mariana C, Limban Carmen, Telehoiu Alexandra, Ioniță Petre
Biochemistry and Catalysis, Department of Organic Chemistry, Faculty of Chemistry, University of Bucharest, 030018 Bucharest, Romania.
Institute of Physical Chemistry, 202 Spl. Independentei, 060021 Bucharest, Romania.
Materials (Basel). 2018 Sep 13;11(9):1704. doi: 10.3390/ma11091704.
(1) Background: Graphene oxide is a new carbon-based material that contains functional groups (carboxyl, hydroxyl, carbonyl, epoxy) and therefore can be easily functionalized with organic compounds of interest, yielding hybrid materials with important properties and applications. (2) Methods: Graphene oxide has been obtained by a modified Hummers method and activated by thionyl chloride in order to be covalently functionalized with amines. Thus obtained hybrid materials were characterized by infrared and Raman spectroscopy, elemental analysis and scanning electron microscopy and then tested for their antimicrobial and anti-biofilm activity. (3) Results: Eight amines of interest were used to functionalize grapheme oxide and the materials thus obtained were tested against Gram-negative (, ) and Gram-positive () bacterial strainsin plankonic and biofilm growth state. Both amines, as well as the functionalized materials, exhibited anti-microbial features. Three to five functionalized graphene oxide materials exhibited improved inhibitory activity against planktonic strains as compared with the respective amines. In exchange, the amines alone proved generally more efficient against biofilm-embedded cells. (4) Conclusions: Such hybrid materials may have a wide range of potential use in biomedical applications.
(1) 背景：氧化石墨烯是一种新型碳基材料，含有官能团（羧基、羟基、羰基、环氧基），因此能够很容易地与目标有机化合物发生官能化反应，生成具有重要性质和应用的杂化材料。(2) 方法：采用改进的Hummers法制备氧化石墨烯，并通过亚硫酰氯进行活化，以便与胺类进行共价官能化反应。通过红外光谱、拉曼光谱、元素分析和扫描电子显微镜对由此获得的杂化材料进行表征，然后测试其抗菌和抗生物膜活性。(3) 结果：使用八种目标胺类对氧化石墨烯进行官能化，并针对革兰氏阴性（ ， ）和革兰氏阳性（ ）细菌菌株在浮游和生物膜生长状态下对所得材料进行测试。胺类以及官能化材料均表现出抗菌特性。与相应的胺类相比，三到五种官能化氧化石墨烯材料对浮游菌株表现出更高的抑制活性。相反，单独的胺类通常对生物膜包埋细胞更有效。(4) 结论：此类杂化材料在生物医学应用中可能具有广泛的潜在用途。
