基于微流控技术的单细胞研究：现状与未来展望。

Department of Pathology, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.

Department of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.

Molecules. 2018 Sep 13;23(9):2347. doi: 10.3390/molecules23092347.

Investigation of cell behavior under different environments and manual operations can give information in specific cellular processes. Among all cell-based analysis, single-cell study occupies a peculiar position, while it can avoid the interaction effect within cell groups and provide more precise information. Microfluidic devices have played an increasingly important role in the field of single-cell study owing to their advantages: high efficiency, easy operation, and low cost. In this review, the applications of polymer-based microfluidics on cell manipulation, cell treatment, and cell analysis at single-cell level are detailed summarized. Moreover, three mainly types of manufacturing methods, i.e., replication, photodefining, and soft lithography methods for polymer-based microfluidics are also discussed.

研究细胞在不同环境和人工操作下的行为可以提供特定细胞过程的信息。在所有基于细胞的分析中，单细胞研究占据着特殊的地位，因为它可以避免细胞群体内的相互作用，并提供更精确的信息。微流控器件由于其高效、易操作和低成本的优点，在单细胞研究领域发挥了越来越重要的作用。在这篇综述中，详细总结了基于聚合物的微流控技术在单细胞水平上的细胞操作、细胞处理和细胞分析中的应用。此外，还讨论了基于聚合物的微流控三种主要制造方法，即复制、光定义和软光刻方法。