Lurain N S, Thompson K D, Farrand S K
J Clin Microbiol. 1986 Nov;24(5):724-30. doi: 10.1128/jcm.24.5.724-730.1986.
A method for rapid identification of human cytomegalovirus (HCMV) was developed with biotinylated DNA probes. BamHI restriction fragments from HCMV strain AD169 were selected and tested for their ability to detect virus in patient urine samples. All probes detected 30 pg of HCMV AD169 DNA. The BamHI B fragment detected 15 of 29 cell-culture-positive samples (sensitivity, 52%). There were four samples which were probe positive and cell culture negative (specificity, 87%). The D and H fragments used as combined probes detected 17 of 21 cell-culture-positive samples (sensitivity, 81%). There were five probe-positive and cell-culture-negative samples (specificity, 68.8%). The H fragment, when used alone, detected 11 of 14 culture-positive samples, and 5 samples were culture negative and probe positive. Sensitivity (78.6%) and specificity (76.2%) for the H fragment were similar to those for the combined probes, but the color intensity of the positive reactions detected by the H fragment alone was lower. There was unexpected cross-reactivity with herpes simplex type 1 and 2 controls when the combined D and H probes were used. Specific hybridization was demonstrated between subfragments of the HCMV BamHI D fragment and the herpes simplex virus type 1 EcoRI M fragment.
利用生物素化DNA探针开发了一种快速鉴定人巨细胞病毒（HCMV）的方法。从HCMV AD169株中选择BamHI限制性片段，并检测其在患者尿液样本中检测病毒的能力。所有探针均可检测到30 pg的HCMV AD169 DNA。BamHI B片段在29个细胞培养阳性样本中检测到15个（敏感性为52%）。有4个样本探针阳性但细胞培养阴性（特异性为87%）。用作联合探针的D和H片段在21个细胞培养阳性样本中检测到17个（敏感性为81%）。有5个探针阳性且细胞培养阴性的样本（特异性为68.8%）。单独使用H片段时，在14个培养阳性样本中检测到11个，有5个样本培养阴性但探针阳性。H片段的敏感性（78.6%）和特异性（76.2%）与联合探针相似，但单独使用H片段检测到的阳性反应的颜色强度较低。使用联合D和H探针时，与1型和2型单纯疱疹病毒对照出现了意外的交叉反应。在HCMV BamHI D片段的亚片段与1型单纯疱疹病毒EcoRI M片段之间证明了特异性杂交。
