Midthun K, Hoshino Y, Kapikian A Z, Chanock R M
J Clin Microbiol. 1986 Nov;24(5):822-6. doi: 10.1128/jcm.24.5.822-826.1986.
A series of reassortants was isolated from coinfection of cell cultures with wild-type bovine rotavirus (UK strain [serotype 6]) or rhesus rotavirus (strain MMU18006 [serotype 3]) and a tissue culture-adapted human rotavirus strain, ST3 (serotype 4). Monospecific antiserum or a set of monoclonal antibodies to the major outer capsid neutralization glycoprotein, VP7, of the animal rotavirus parent was used to select for reassortants with human rotavirus serotype 4 neutralization specificity. The majority of reassortants contained only gene 9 of the human rotavirus parent, ST3, whereas the remaining genes were derived from the animal rotavirus parent. These single human rotavirus gene substitution reassortants were neutralized to high titer by hyperimmune serum directed at ST3, thus demonstrating that gene 9 of ST3 codes for the major neutralization protein of this strain. Moreover, these single gene substitution, reassortants were also neutralized to low titer by antiserum directed at their animal rotavirus parent, probably because they derived gene 4, which codes for another outer capsid protein, VP3, from their animal rotavirus parent. None of the reassortants derived gene 4, which had previously been shown to be responsible for host range restriction of human rotaviruses in tissue culture, from ST3, despite the fact that the ST3 strain used for gene reassortment had been tissue culture adapted.
通过将细胞培养物与野生型牛轮状病毒（英国毒株[血清型6]）或恒河猴轮状病毒（MMU18006毒株[血清型3]）以及一种适应组织培养的人轮状病毒毒株ST3（血清型4）共同感染，分离出了一系列重配病毒。使用针对动物轮状病毒亲本主要外衣壳中和糖蛋白VP7的单特异性抗血清或一组单克隆抗体，来筛选具有人轮状病毒血清型4中和特异性的重配病毒。大多数重配病毒仅含有亲本人轮状病毒ST3的基因9，而其余基因则来自动物轮状病毒亲本。这些单一人轮状病毒基因替代重配病毒被针对ST3的超免疫血清高效中和，从而表明ST3的基因9编码该毒株的主要中和蛋白。此外，这些单基因替代重配病毒也被针对其动物轮状病毒亲本的抗血清低效中和，这可能是因为它们从动物轮状病毒亲本获得了编码另一种外衣壳蛋白VP3的基因4。尽管用于基因重配的ST3毒株已适应组织培养，但没有一个重配病毒从ST3获得此前已证明在组织培养中负责人类轮状病毒宿主范围限制的基因4。
