Snodgrass D R, Hoshino Y, Fitzgerald T A, Smith M, Browning G F, Gorziglia M
Moredun Research Institute, Edinburgh, U.K.
J Gen Virol. 1992 Sep;73 ( Pt 9):2319-25. doi: 10.1099/0022-1317-73-9-2319.
A series of five reassortant viruses each containing the VP4 gene of a distinct bovine rotavirus and the VP7 gene of human rotavirus strain ST3 was prepared, and antisera to these were produced in rabbits. In neutralization tests, these antisera allowed the differentiation of the five original strains (from three different VP7 or G serotypes) into three or possibly four VP4 or P serotypes. All of a further seven bovine rotavirus strains adapted to cell culture were successfully typed by these antisera. There was a degree of cross-reaction between antiserum to the fourth bovine rotavirus P serotype and the predominant human rotavirus serotype. However, antisera raised in guinea-pigs to recombinant VP4 from this serotype showed the bovine serotype to be distinct. There was no significant serological relationship between these four bovine rotavirus P serotypes and previously described P serotypes from rotaviruses isolated from man and non-bovine animals. The predominant bovine rotavirus VP7 serotypes G6 and G10 tended to have distinct P serotypes also.
制备了一系列五种重配病毒，每种病毒都含有一种独特的牛轮状病毒的VP4基因和人轮状病毒ST3株的VP7基因，并在兔体内产生了针对这些病毒的抗血清。在中和试验中，这些抗血清能够将五种原始毒株（来自三种不同的VP7或G血清型）区分为三种或可能四种VP4或P血清型。另外七种适应细胞培养的牛轮状病毒毒株也通过这些抗血清成功分型。针对第四种牛轮状病毒P血清型的抗血清与主要的人轮状病毒血清型之间存在一定程度的交叉反应。然而，用豚鼠制备的针对该血清型重组VP4的抗血清显示该牛血清型是独特的。这四种牛轮状病毒P血清型与先前从人和非牛动物分离的轮状病毒中描述的P血清型之间没有显著的血清学关系。主要的牛轮状病毒VP7血清型G6和G10也往往具有不同的P血清型。
