Sansonno D E, Detomaso P, Papanice M A, Manghisi O G
J Immunol Methods. 1986 Oct 23;93(1):71-6. doi: 10.1016/0022-1759(86)90434-5.
Receptors for polymerised human albumin (pHSA-Rs) were detected in unfixed cryostat sections from HBsAg chronic carriers using direct immunoperoxidase and immunoadherence methods. Although pHSA-Rs were detected by both methods, the receptors detected by immunoperoxidase were associated with HBV and showed properties different from the receptors detected by immunoadherence. The double immunocytochemical staining which detected contemporaneously pHSA-Rs and HBsAg in the same cell showed that there are two types of infected hepatocytes: one capable of synthesizing pHSA-Rs and HBsAg and the other capable of synthesizing only HBsAg. The intrahepatocyte synthesis of pHSA-Rs does not correlate with the severity of chronic liver disease or with the presence of tissue HB core antigen.
采用直接免疫过氧化物酶法和免疫黏附法，在乙肝表面抗原（HBsAg）慢性携带者未固定的低温恒温器切片中检测到聚合人白蛋白受体（pHSA-Rs）。虽然两种方法均检测到了pHSA-Rs，但免疫过氧化物酶法检测到的受体与乙肝病毒（HBV）相关，且表现出与免疫黏附法检测到的受体不同的特性。在同一细胞中同时检测pHSA-Rs和HBsAg的双重免疫细胞化学染色显示，存在两种类型的受感染肝细胞：一种能够合成pHSA-Rs和HBsAg，另一种仅能合成HBsAg。肝内pHSA-Rs的合成与慢性肝病的严重程度或组织乙肝核心抗原的存在无关。
