Li Lan, Yu Xiaoming, Zhang Haoming, Cheng Haiwei, Hou Liting, Zheng Qisheng, Hou Jibo
National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, Jiangsu, China.
Virus Genes. 2019 Apr;55(2):174-181. doi: 10.1007/s11262-019-01633-7. Epub 2019 Jan 14.
Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin's inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin's action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.
格里菲斯菌素是一种对包膜病毒具有强大抗病毒活性的凝集素。本研究的目的是评估格里菲斯菌素对猪流行性腹泻病毒（PEDV）的抑制作用。结果表明，格里菲斯菌素使Vero细胞的PEDV感染减少了约82.8%。此外，通过添加时间试验和逆转录定量聚合酶链反应（RT-qPCR），我们发现延迟添加格里菲斯菌素对PEDV的抑制作用比早期处理弱。格里菲斯菌素对PEDV的作用机制包括阻止病毒附着于宿主细胞和破坏细胞间传播；其双重作用模式使格里菲斯菌素与大多数其他抗病毒药物有所不同。总之，格里菲斯菌素被确定为一种有效的PEDV抑制剂，可能是预防PEDV感染的候选药物。
