格里菲斯素与日本脑炎病毒的糖基化蛋白（E蛋白和prM蛋白）结合，并抑制其感染。
Griffithsin binds to the glycosylated proteins (E and prM) of Japanese encephalitis virus and inhibit its infection.
作者信息
Ishag Hassan Z A, Li Chen, Wang Fengjuan, Mao Xiang
机构信息
College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, Jiangsu Province, China; College of Veterinary Sciences, Nyala University, Nyala, Sudan.
College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, Jiangsu Province, China.
出版信息
Virus Res. 2016 Apr 2;215:50-4. doi: 10.1016/j.virusres.2016.01.016. Epub 2016 Jan 25.
Griffithsin (GRFT) is a broad-spectrum antiviral protein against several glycosylated viruses. In our previous publication, we have shown that GRFT exerted antiviral activity against Japanese encephalitis virus (JEV) infection. Herein, we further elucidated the mechanism by which GRFT inhibits JEV infection in BHK-21 cells. In vitro experiments using Pull-down assay and Co-immunoprecipitation (CO-IP) assay showed that GRFT binds to the JEV glycosylated viral proteins, specifically the enveloped (E) and premature (prM) glycoproteins. The binding of GRFT to the JEV was competitively inhibited by increasing concentrations of mannose; in turns abolished anti-JEV activity of GRFT. We suggested that, the binding of GRFT to the glycosylated viral proteins may contribute to its anti-JEV activity. Collectively, our data indicated a possible mechanism by which GRFT exerted its anti-JEV activity. This observation suggests GRFT's potentials in the development of therapeutics against JEV or other flavivirus infection.
格里菲斯菌素（GRFT）是一种针对多种糖基化病毒的广谱抗病毒蛋白。在我们之前的出版物中，我们已经表明GRFT对日本脑炎病毒（JEV）感染具有抗病毒活性。在此，我们进一步阐明了GRFT抑制BHK - 21细胞中JEV感染的机制。使用下拉分析和免疫共沉淀（CO - IP）分析的体外实验表明，GRFT与JEV糖基化病毒蛋白结合，特别是包膜（E）糖蛋白和前体（prM）糖蛋白。随着甘露糖浓度的增加，GRFT与JEV的结合受到竞争性抑制；进而消除了GRFT的抗JEV活性。我们认为，GRFT与糖基化病毒蛋白的结合可能有助于其抗JEV活性。总体而言，我们的数据表明了GRFT发挥其抗JEV活性的一种可能机制。这一观察结果表明GRFT在开发针对JEV或其他黄病毒感染的治疗方法方面具有潜力。
Zotero 插件