Najar Ishfaq Nabi, Sherpa Mingma Thundu, Das Sayak, Verma Kamalesh, Dubey Vikash Kumar, Thakur Nagendra
1Department of Microbiology, School of Life Sciences, Sikkim University, 6th Mile, Samdur, Tadong, Gangtok - 737102, Sikkim, India.
2Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati-781039, Assam, India.
Int J Syst Evol Microbiol. 2018 Nov;68(11):3430-3434. doi: 10.1099/ijsem.0.003002. Epub 2018 Sep 17.
A thermophilic, spore-forming, rod-shaped bacterium isolated from the Yumthang hot spring in North Sikkim, India was subjected to taxonomic studies. The thermophilic bacterial isolate was designated as strain AYN2. Cells were Gram-stain-positive, aerobic, motile, rod-shaped, catalase-positive and methyl red-negative. Strain AYN2 was able to grow in the pH range from 6 to 10 (optimum, pH 7.5-8.0), at 40-70 °C (60 °C) and in NaCl concentrations of 0-4 % (1 %). The major cellular fatty acids were iso-C15 : 0 (12.8 %), iso-C16 : 0 (13.9 %) and iso-C17 : 0 (13.8 %). No matches were found in the rtsba6 Sherlock libraries. The G+C content of the genomic DNA was 42.11 mol%. Based on phylogenetic analysis of the 16S rRNA gene sequences, strain AYN showed highest sequence similarity to the type strain of Geobacillus toebii (96 %). However, the phenotypic properties of strain AYN2 were clearly distinct from those of G. toebii and related species. On the basis of polyphasic analysis, strain AYN2 represents a novel species in the genus Geobacillus, for which the name Geobacillus yumthangensis sp. nov. is proposed. The type strain is AYN2(MTCC=12749=KCTC=33950= JCM 32596).
从印度北锡金邦的尤姆唐温泉中分离出的一株嗜热、产芽孢、杆状细菌进行了分类学研究。该嗜热细菌分离株被命名为AYN2菌株。细胞革兰氏染色阳性，好氧，具运动性，杆状，过氧化氢酶阳性，甲基红阴性。AYN2菌株能够在pH值6至10（最适pH值7.5 - 8.0）、40 - 70°C（60°C）以及NaCl浓度0 - 4%（1%）的条件下生长。主要的细胞脂肪酸为异C15:0（12.8%）、异C16:0（13.9%）和异C17:0（13.8%）。在rtsba6 Sherlock文库中未找到匹配项。基因组DNA的G + C含量为42.11 mol%。基于16S rRNA基因序列的系统发育分析，AYN菌株与嗜热栖热放线杆菌模式菌株的序列相似性最高（96%）。然而，AYN2菌株的表型特征与嗜热栖热放线杆菌及相关物种明显不同。基于多相分析，AYN2菌株代表嗜热栖热放线杆菌属中的一个新物种，为此提出新名称嗜热栖热尤姆唐杆菌（Geobacillus yumthangensis sp. nov.）。模式菌株为AYN2（MTCC = 12749 = KCTC = 33950 = JCM 32596）。
