Sherpa Mingma Thundu, Das Sayak, Najar Ishfaq Nabi, Thakur Nagendra
Department of Microbiology, School of Life Sciences, Sikkim University, 6th Mile, Samdur Gangtok, Sikkim 737102, India.
Curr Res Microb Sci. 2020 Oct 15;2:100012. doi: 10.1016/j.crmicr.2020.100012. eCollection 2021 Dec.
strain P13 was isolated from the Kanchengayao glacier's accumulation zone. A comprehensive study was done on this strain as it produced protease enzyme and thus having industrial potential. The whole genome sequence, FAME, morphological and biochemical characteristics of the strain P13 is being presented. The genome of P13 strain possesses 2,689,565 total reads, with an average + content of 69.9%. The whole-genome assembly is having 548 contigs (with PEGs). The genome contains 2,985 coding sequences with 55 rRNA predicted genes. More than 88% of the total pre-processed reads from samples were mapped. The identified coding proteins were classified into 27 functional categories based on COG classification. The genome was found to possess genes for ammonium assimilation, galactosylceramide and sulfatide metabolism. The major enzymes present are beta-galactosidase, thiol peroxidase, thiolredoxin reductase, glutamate synthase, and glutamate-ammonia-ligase adenyltransferase. The genome information of P13 provides the basis for understanding the functional properties and abilities to act as a potential cold-active enzyme producer and nitrogen-fixing bacteria.
菌株P13是从坎城盖尧冰川的积累区分离出来的。由于该菌株能产生蛋白酶,具有工业潜力,因此对其进行了全面研究。本文介绍了菌株P13的全基因组序列、脂肪酸甲酯分析、形态学和生化特性。P13菌株的基因组共有2,689,565条 reads,平均GC含量为69.9%。全基因组组装有548个重叠群(含PEGs)。该基因组包含2,985个编码序列和55个预测的rRNA基因。样本中超过88%的预处理reads被定位。根据COG分类,鉴定出的编码蛋白被分为27个功能类别。发现该基因组拥有铵同化、半乳糖神经酰胺和硫脂代谢的基因。存在的主要酶有β-半乳糖苷酶、硫醇过氧化物酶、硫氧还蛋白还原酶、谷氨酸合酶和谷氨酸-氨连接酶腺苷转移酶。P13的基因组信息为理解其功能特性以及作为潜在的冷活性酶生产者和固氮细菌的能力提供了基础。