Schuette S A, Rose R C
Nutr Cancer. 1986;8(4):257-65. doi: 10.1080/01635588609513902.
The possibility that long-term feeding of diets high in fat or fiber could alter the colonic mucosa and subsequent colonic absorption of 1,2-dimethylhydrazine (DMH) in situ was examined in the rat model. Male Sprague-Dawley rats were fed one of four experimental diets for six weeks prior to studies of DMH absorption and bile acid excretion; dietary treatments consisted of two levels of fat (12 and 47% of calories from corn oil) fed at each of two levels of fiber (plus or minus 15% wheat bran). Two sets of DMH absorption studies (Studies 1 and 2) were performed; the first used a 10- and the second a 20-minute test period. In Study 1, DMH absorption was greater in those animals that had been fed the high level of corn oil when additional fiber was not present in the diet. When a longer absorption period was used (Study 2), this effect of diet on DMH absorption was not apparent. The level of fiber, not the fat intake, altered bile acid excretion. Bile acid concentration (mg/g dry wt) decreased with added fiber, whereas total bile acid excretion (mg/day) increased. These results indicate that high levels of dietary fat may result in small increases in DMH absorption which are unrelated to changes in bile acid concentration.
在大鼠模型中，研究了长期喂食高脂肪或高纤维饮食是否会改变结肠黏膜以及随后原位结肠对1,2 - 二甲基肼（DMH）的吸收情况。在进行DMH吸收和胆汁酸排泄研究之前，雄性斯普拉格 - 道利大鼠被喂食四种实验饮食之一六周；饮食处理包括两种脂肪水平（玉米油热量的12%和47%），每种脂肪水平又分别搭配两种纤维水平（加或不加15%的麦麸）。进行了两组DMH吸收研究（研究1和研究2）；第一个研究采用10分钟的测试期，第二个研究采用20分钟的测试期。在研究1中，当饮食中不存在额外纤维时，喂食高水平玉米油的动物对DMH的吸收更高。当使用更长的吸收期（研究2）时，饮食对DMH吸收的这种影响并不明显。是纤维水平而非脂肪摄入量改变了胆汁酸排泄。添加纤维后，胆汁酸浓度（毫克/克干重）降低，而总胆汁酸排泄量（毫克/天）增加。这些结果表明，高膳食脂肪水平可能会使DMH吸收略有增加，且这与胆汁酸浓度的变化无关。
