一种对农杆菌毒力至关重要的基因与一组正向调控基因座家族同源。
A gene essential for Agrobacterium virulence is homologous to a family of positive regulatory loci.
作者信息
Winans S C, Ebert P R, Stachel S E, Gordon M P, Nester E W
出版信息
Proc Natl Acad Sci U S A. 1986 Nov;83(21):8278-82. doi: 10.1073/pnas.83.21.8278.
Abstract
The vir region of Agrobacterium tumefaciens spans at least six transcriptional loci required for crown gall tumorigenesis. The transcriptional induction of two of these vir loci in response to cocultivation with tobacco suspension cells was measured by using bacteria containing mutations in each of the six vir loci located on the Ti plasmid. Induction of these vir genes occurred only in bacteria that had functional copies of virA and virG. The nucleic acid sequence of a 1.25-kilobase clone encompassing virG contains one open reading frame capable of coding for a protein of about 30,000 daltons. The amino acid sequence of the predicted virG product is homologous to that of eight bacterial proteins, including that of the ompR gene of Escherichia coli. Most, although not all, of these proteins, like VirG, are positive regulatory elements.
摘要
根癌土壤杆菌的vir区域至少跨越六个与冠瘿瘤形成相关的转录位点。通过使用含有位于Ti质粒上六个vir位点中每个位点突变的细菌，测定了其中两个vir位点在与烟草悬浮细胞共培养时的转录诱导情况。这些vir基因的诱导仅发生在具有virA和virG功能拷贝的细菌中。一个包含virG的1.25千碱基克隆的核酸序列含有一个开放阅读框，能够编码一种约30,000道尔顿的蛋白质。预测的virG产物的氨基酸序列与八种细菌蛋白的氨基酸序列同源，包括大肠杆菌ompR基因的氨基酸序列。这些蛋白中的大多数（尽管不是全部），如VirG，都是正调控元件。
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