Bellinzoni R C, Mattion N, La Torre J L, Scodeller E A
Res Vet Sci. 1987 Mar;42(2):257-9.
Faecal samples were collected from 177 diarrhoeic and 40 healthy calves from 19 farms in Buenos Aires State, Argentina during 1984 and 1985. Samples were examined for rotavirus by enzyme-linked immunosorbent assay (ELISA) and polyacrylamide gel electrophoresis (PAGE) of their genomic RNA segments. Rotavirus was found in 95 samples of diarrhoeic calves (53 per cent) and in three healthy calves (7 per cent). All positive samples were tentatively classified as group A on the basis of electropherotype and ELISA test reactivity and exhibited 18 different genomic electropherotypic patterns.
1984年至1985年期间,从阿根廷布宜诺斯艾利斯省19个农场的177头腹泻犊牛和40头健康犊牛采集粪便样本。通过酶联免疫吸附测定(ELISA)和对其基因组RNA片段进行聚丙烯酰胺凝胶电泳(PAGE)检测样本中的轮状病毒。在95份腹泻犊牛样本(53%)和3份健康犊牛样本(7%)中发现了轮状病毒。根据电泳图谱和ELISA试验反应性,所有阳性样本初步分类为A组,并呈现出18种不同的基因组电泳图谱模式。