School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Anal Chim Acta. 2018 Dec 4;1035:168-174. doi: 10.1016/j.aca.2018.06.039. Epub 2018 Jun 15.
A novel chemiluminescence immunoassay based on luminol-modified gold nanoclusters (AuNCs@Peps@luminol) was developed for simultaneous detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA-DHEE) and tetrabromobisphenol A mono(hydroxyethyl) ether (TBBPA-MHEE), an important derivative and byproduct of tetrabromobisphenol A (TBBPA), respectively. In the system, alkaline phosphatase (ALP) was labeled on the second antibody (Ab) for signal amplification. When ALP-Ab was captured by antigen-primary antibody (Ab) complex, disodium phenyl phosphate (PPNa) generated massive phenol under the catalysis of ALP, markedly inhibiting the chemiluminescence intensity of AuNCs@Peps@luminol. Under the optimized conditions, the calculated detection of limit (LOD, 90% inhibition) was 0.078 μg/L for TBBPA-DHEE with a linear range of 0.23-9.32 μg/L, which was lower 9 times than that of conventional ELISA with the same antibody. In addition, our method showed satisfactory accuracy and precision (recoveries, 88.00-113.4%; CV, 2.75-8.14%), it can be applied to systematically investigate the concentration of the trace TBBPA-DHEE and TBBPA-MHEE in environmental and food samples.
基于鲁米诺修饰的金纳米簇（AuNCs@Peps@luminol）的新型化学发光免疫分析被开发用于同时检测四溴双酚 A 双（2-羟乙基）醚（TBBPA-DHEE）和四溴双酚 A 单（2-羟乙基）醚（TBBPA-MHEE），它们分别是四溴双酚 A（TBBPA）的重要衍生物和副产物。在该体系中，碱性磷酸酶（ALP）被标记在第二抗体（Ab）上用于信号放大。当 ALP-Ab 被抗原-一抗（Ab）复合物捕获时，在 ALP 的催化下，大量的酚被生成，显著抑制了 AuNCs@Peps@luminol 的化学发光强度。在优化条件下，TBBPA-DHEE 的计算检测限（LOD，90%抑制）为 0.078μg/L，线性范围为 0.23-9.32μg/L，比相同抗体的常规 ELISA 低 9 倍。此外，我们的方法表现出令人满意的准确性和精密度（回收率，88.00-113.4%；CV，2.75-8.14%），可用于系统地研究环境和食品样品中痕量 TBBPA-DHEE 和 TBBPA-MHEE 的浓度。
