School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.
Nanjing Institute of Environmental Science, Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Ministry of Ecology and Environment, Nanjing, 210042, China.
Anal Bioanal Chem. 2020 Jun;412(15):3673-3681. doi: 10.1007/s00216-020-02604-w. Epub 2020 Apr 8.
The environmental concentration of tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA-DHEE) and tetrabromobisphenol A mono(hydroxyethyl) ether (TBBPA-MHEE), as some of the main derivatives and byproducts of tetrabromobisphenol A (TBBPA), are obscure due to lacking available analytical methods. In the present study, a multiplexed competitive chemiluminescent imaging immunoassay (CLIIA) based on specific monoclonal antibody respectively against TBBPA-MHEE and TBBPA-DHEE has been developed for the simultaneous detection of the two targets. To improve the sensitivity of immunoassay, Au nanoparticles (AuNPs) were utilized as solid support for loading horseradish peroxidase, labeled to obtain the multi-enzyme particles. Under the optimized conditions, the limits of detection (LODs) for TBBPA-MHEE and TBBPA-DHEE were 1.85 ng/mL and 2.05 ng/mL, respectively. After estimating its accuracy and precision, the established method was applied for investigation of the contaminants at the inner rivers in Zhenjiang, Jiangsu Province. Our results indicated that no TBBPA-MHEE was found in all 11 water samples and two samples of TBBPA-DHEE were detected up to 6.34 ng/mL, possibly ascribed to the dense population and slow flow rate around the sampling site. Graphical abstract.
由于缺乏可用的分析方法，四溴双酚 A 双（2-羟乙基）醚（TBBPA-DHEE）和四溴双酚 A 单（2-羟乙基）醚（TBBPA-MHEE）作为四溴双酚 A（TBBPA）的一些主要衍生物和副产物，其环境浓度尚不清楚。在本研究中，基于针对 TBBPA-MHEE 和 TBBPA-DHEE 的特异性单克隆抗体，建立了一种多重竞争化学发光免疫分析（CLIIA），用于同时检测这两种靶标。为了提高免疫分析的灵敏度，利用金纳米粒子（AuNPs）作为负载辣根过氧化物酶的固体载体，标记后获得多酶颗粒。在优化条件下，TBBPA-MHEE 和 TBBPA-DHEE 的检出限（LOD）分别为 1.85 ng/mL 和 2.05 ng/mL。在评估其准确性和精密度后，该方法用于调查江苏省镇江市内河流的污染物。我们的结果表明，在所有 11 个水样中均未发现 TBBPA-MHEE，而在两个 TBBPA-DHEE 样品中检测到的浓度高达 6.34 ng/mL，这可能是由于采样点周围人口密集且流速缓慢。
