High-throughput chemiluminescence immunoassay based on Co/hemin synergistic catalysis for sensitive detection tetrabromobisphenol A bis(2-hydroxyethyl) ether in the environments.

Here, a novel chemiluminescence (CL) immunoassay was fabricated for sensitive determination of tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA-DHEE), one of typical tetrabromobisphenol A derivatives. At the indirectly competitive method, the synthesized PS@hemin@Co was labelled by secondary antibody (Ab) instead of common natural enzymes, which showed excellent catalysis towards the decomposition of luminol-HO for producing CL signal. Furthermore, the CL signal was greatly amplified owing to the synergistic catalysis of hemin and Co in the detection system. Under the optimized conditions, the established method offered (i) low detection limit (LOD, 0.9 μg/L), which was almost 5 times lower than that using a conventional ELISA with the same antibody; (ii) a good linearity (1.6-14.3 μg/L); (iii) satisfactory accuracy and precision (recoveries, 89.67-125.33%; CV, 2.75-8.37%). The proposed CL immunoassay was applied for analysis of environmental samples from various sources collected from Jiangsu and Zhejiang province, China. And the detected concentrations were ranged in 2.4-3.7 μg/L in environmental waters and 1.8-2.4 ng/g (dry weight, dw) in soil samples, indicating great potential for trace TBBPA-DHEE detection from environmental samples.