Pater M M, Dunne J, Hogan G, Ghatage P, Pater A
Virology. 1986 Nov;155(1):13-8. doi: 10.1016/0042-6822(86)90163-7.
A total of 100 colposcopic biopsies from patients with abnormal Papanicolau's tests were surveyed for the presence of human papillomavirus (HPV) types 16 and 18 sequences by spot-blot hybridization. HPV 16 and 18 DNA sequences were detected in 58% of the biopsies. None of the cervical intraepithelial neoplasia grade I (CIN I) contained HPV 16 while 50% of the CIN III lesions (carcinoma in situ, CIS) contained HPV 16. HPV 18-related sequences were equally represented in CIN I, II, and III. Southern-blot hybridization of total undigested cellular DNA revealed the presence of HPV DNA sequences only in an episomal form. While the restriction enzyme patterns in HPV 16-positive samples were mostly identical to the originally cloned sequence, the restriction enzyme pattern for HPV 18-positive samples were different from that of HPV 18 but identical to each other. Furthermore, this DNA hybridized more strongly to HPV 18 under nonstringent conditions, suggesting a new type.
通过斑点杂交法，对100例巴氏试验异常患者的阴道镜活检样本进行检测，以确定是否存在人乳头瘤病毒（HPV）16型和18型序列。在58%的活检样本中检测到HPV 16和18 DNA序列。宫颈上皮内瘤变I级（CIN I）中未发现HPV 16，而CIN III级病变（原位癌，CIS）中有50%含有HPV 16。HPV 18相关序列在CIN I、II和III中分布相同。对未消化的细胞总DNA进行Southern杂交，结果显示HPV DNA序列仅以游离形式存在。HPV 16阳性样本的限制性内切酶图谱大多与最初克隆的序列相同，而HPV 18阳性样本的限制性内切酶图谱与HPV 18不同，但彼此相同。此外，在非严格条件下，该DNA与HPV 18的杂交更强，提示为一种新型。
