Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands.
Department of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.
Nat Methods. 2018 Oct;15(10):781-784. doi: 10.1038/s41592-018-0136-6. Epub 2018 Sep 17.
Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.
方法融合了单个结构的多个定位显微镜图像，可以提高信噪比和分辨率，但它们通常受到模板偏差或对配准误差的敏感性的影响。我们提出了一种基于全对全配准的无模板粒子融合方法，该方法对个别配准错误和标记不足具有鲁棒性。我们通过融合 383 个 DNA 折纸纳米结构（标记密度为 80%）实现了 3.3nm 的傅立叶环相关（FRC）图像分辨率，对于标记密度为 30%的结构，分辨率达到 5.0nm。
