Recent studies have shown that the representation of an item in visual working memory (VWM) can bias the deployment of attention to stimuli in the visual scene possessing the same features. When multiple item representations are simultaneously held in VWM, whether these representations, especially those held in a non-prioritized or accessory status, are able to bias attention, is still controversial. In the present study we adopted an eye tracking technique to shed light on this issue. In particular, we implemented a manipulation aimed at prioritizing one of the VWM representation to an active status, and tested whether attention could be guided by both the prioritized and the accessory representations when they reappeared as distractors in a visual search task. Notably, in Experiment 1, an analysis of first fixation proportion (FFP) revealed that both the prioritized and the accessory representations were able to capture attention suggesting a significant attentional guidance effect. However, such effect was not present in manual response times (RT). Most critically, in Experiment 2, we used a more robust experimental design controlling for different factors that might have played a role in shaping these findings. The results showed evidence for attentional guidance from the accessory representation in both manual RTs and FFPs. Interestingly, FFPs showed a stronger attentional bias for the prioritized representation than for the accessory representation across experiments. The overall findings suggest that multiple VWM representations, even the accessory representation, can simultaneously interact with visual attention.