Zhao B, Mackow E, Buckler-White A, Markoff L, Chanock R M, Lai C J, Makino Y
Virology. 1986 Nov;155(1):77-88. doi: 10.1016/0042-6822(86)90169-8.
DNA sequences (approximately 11,000 nucleotides) representing the full-length genome of the dengue virus type 4 were cloned. The sequence of the first 2,429 nucleotides at the 5' terminus which includes the coding region for the structural proteins is presented. The virion structural proteins are encoded in one long open reading frame specifying a polyprotein precursor which is apparently proteolytically cleaved by a mechanism resembling that proposed for expression of structural proteins of other flaviviruses such as yellow fever (YF) and West Nile (WN) viruses. The N terminus for each of the dengue virus structural proteins was tentatively assigned by homology alignment to the corresponding sequence of YF or WN virus. Comparison of sequence homology of structural proteins suggests that dengue virus is more closely related to WN virus than to YF virus or Murray Valley encephalitis virus. Finally, analysis of the extreme 5'- and 3'-terminal nucleotides of the dengue virus genome revealed sequences that may be involved in transcription, replication, and packaging of viral RNA.
代表登革4型病毒全长基因组的DNA序列（约11,000个核苷酸）被克隆。呈现了5'端前2,429个核苷酸的序列，其中包括结构蛋白的编码区。病毒体结构蛋白由一个长开放阅读框编码，该阅读框指定了一个多蛋白前体，该前体显然通过一种类似于黄热病（YF）和西尼罗河（WN）病毒等其他黄病毒结构蛋白表达所提出的机制进行蛋白水解切割。通过同源性比对，将登革病毒各结构蛋白的N端初步定位到YF或WN病毒的相应序列。结构蛋白序列同源性比较表明，登革病毒与WN病毒的关系比与YF病毒或墨累谷脑炎病毒的关系更密切。最后，对登革病毒基因组5'和3'末端极端核苷酸的分析揭示了可能参与病毒RNA转录、复制和包装的序列。
