原发性肺癌中 HPV 的存在和活性。
Presence and activity of HPV in primary lung cancer.
机构信息
Laboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Center of Biological Sciences, Federal University of Pernambuco, Avenida Professor Moraes Rego S/N, Recife, PE, 50670-901, Brazil.
Molecular Biology Laboratory, Pediatric Oncohematology Center, University of Pernambuco, Avenida Agamenon Magalhaes S/N, Recife, PE, 50100-130, Brazil.
出版信息
J Cancer Res Clin Oncol. 2018 Dec;144(12):2367-2376. doi: 10.1007/s00432-018-2748-8. Epub 2018 Sep 17.
PURPOSE
Evaluate the presence of human papillomavirus (HPV) in biopsies of Brazilian patients with lung cancer and also the expression of the E6 and E7 oncoproteins. HPV is widely known as an important condition for cervical cancer although evidence today shows it is associated with several other types of cancer and may also be involved in lung cancer development. However, there are some divergences regarding the presence and activity of HPV in lung carcinogenesis.
METHODS
The detection of HPV was performed by PCR, followed by genotype and immunohistochemical evaluation of E6 and E7 HPV type specific, from 63 patients.
RESULTS
HPV was found to be present in 33 of the 63 samples, and types 16 and 18 were detected with frequencies of 81% (27/33) and 19% (6/33), respectively. About the presence of the virus in different histological types of tumors, HPV was detected in squamous cell carcinoma (39.39%), followed by adenocarcinoma (33.33%) and small cell carcinoma (18.18%) and large cell carcinoma (9.1%). The presence of the E6 (antibody anti-HPV 16 and anti-HPV 18) and E7 (antibody anti-HPV 16 and anti-HPV 18) oncoproteins was detected by immunohistochemical stain technique in 28/33 samples and 25/33 samples, respectively.
CONCLUSIONS
Our results suggest that the lung tumor patients have high prevalence of HPV and the virus is not only present but also active in tumor cells. Therefore, the HPV is probably playing a role in lung carcinogenesis.
目的
评估巴西肺癌患者活检组织中人类乳头瘤病毒（HPV）的存在情况，以及 E6 和 E7 癌蛋白的表达情况。HPV 是众所周知的宫颈癌的重要致病因素，尽管目前的证据表明它与多种其他类型的癌症有关，也可能与肺癌的发生有关。然而，关于 HPV 在肺癌发生中的存在和活性存在一些分歧。
方法
通过聚合酶链反应（PCR）检测 HPV，然后对 63 例患者的 HPV 基因型进行检测，并进行 HPV 型 E6 和 E7 特异性的免疫组织化学评价。
结果
在 63 个样本中发现 33 个样本存在 HPV，分别检测到 16 型和 18 型 HPV 的频率为 81%（27/33）和 19%（6/33）。关于病毒在不同组织学类型肿瘤中的存在情况，HPV 存在于鳞状细胞癌（39.39%）中，其次是腺癌（33.33%）、小细胞癌（18.18%）和大细胞癌（9.1%）。通过免疫组织化学染色技术检测到 28/33 个样本存在 E6（抗 HPV 16 和抗 HPV 18 抗体）和 E7（抗 HPV 16 和抗 HPV 18 抗体）癌蛋白，分别为 25/33 个样本。
结论
我们的结果表明，肺癌患者 HPV 感染率较高，病毒不仅存在于肿瘤细胞中，而且具有活性。因此，HPV 可能在肺癌发生中发挥作用。
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