Smith A L, Winograd D F, Burrage T G
Arch Virol. 1986;91(3-4):233-46. doi: 10.1007/BF01314283.
The growth, stability and seroprevalence in laboratory rodents of the two known strains of mouse adenovirus were compared. The FL strain of mouse adenovirus grew in both L 929 murine fibroblasts and in CMT-93 murine rectal carcinoma cells, whereas the K 87 strain grew only in CMT-93 cells. The bulk of the FL progeny virus was released from the host cells. K 87 virus was largely cell-associated. Both virus strains were stable at 37 degrees C in liquid medium. The K 87 strain was completely inactivated after 5-15 minutes at 56 degrees C, whereas FL infectivity was still detected after two hours at this temperature. Both virus strains were stable in the dessicated state for 14 days, although FL viability was more dependent on the presence of protein in the virus diluent. Seroepidemiologic data suggest that viruses antigenically related to mouse adenovirus are more prevalent among laboratory rats than among laboratory mice and that the virus(es) infecting rats differ from those infecting mice. Results of retrospective serologic testing suggest an association between mouse adenovirus and an outbreak of disease in a mouse breeding colony.
比较了两种已知品系小鼠腺病毒在实验啮齿动物中的生长、稳定性及血清阳性率。小鼠腺病毒的FL品系可在L 929小鼠成纤维细胞和CMT - 93小鼠直肠癌细胞中生长，而K 87品系仅在CMT - 93细胞中生长。大部分FL子代病毒从宿主细胞中释放出来。K 87病毒主要与细胞相关。两种病毒株在液体培养基中于37℃时均稳定。K 87品系在56℃下5 - 15分钟后完全失活，而在此温度下两小时后仍可检测到FL的感染性。两种病毒株在干燥状态下14天均稳定，尽管FL的活力更依赖于病毒稀释液中蛋白质的存在。血清流行病学数据表明，与小鼠腺病毒抗原相关的病毒在实验大鼠中比在实验小鼠中更普遍，且感染大鼠的病毒与感染小鼠的病毒不同。回顾性血清学检测结果表明小鼠腺病毒与一个小鼠繁殖群体中的疾病暴发有关。
