Wigand R, Gelderblom H, Ozel M
Arch Virol. 1977;54(1-2):131-42. doi: 10.1007/BF01314385.
Mouse adenovirus, strain FL (MAV), is best propagated in primary rather than secondary cultures of mouse kidney cells. Virus release from the cells into the medium is fairly efficient; the average yield per cell is about 1000 TCID50. Morphological and other biophysical characteristics are those of a typical adenovirus: the icosahedral shell with a diameter of 74 nm, fiber projections of 29 nm length, its localization inside the infected kidney cells, a buoyant density in CsC1 of 1.34 g/ml, inhibition of multiplication by inhibitors of DNA synthesis. The thermostability is higher than that of human adenoviruses, whereas, in contrast to these, MAV is inactivated to a great extent by trypsin. Complete or incomplete hemagglutinin or toxin-like activity were not detected. The virus shows no cross-neutralization with human adenoviruses and a one-side cross-reaction with another murine adenovirus strain K-87. Soluble complement-fixing antigen of MAX exhibits a sedimentation rate of 12S identical to the hexon component of human adenoviruses; both antigens show a partial antigenic relationship in tests with appropriate antisera.
小鼠腺病毒FL株（MAV）在小鼠肾细胞的原代培养而非传代培养中增殖效果最佳。病毒从细胞释放到培养基中的效率相当高；每个细胞的平均产量约为1000个半数组织培养感染剂量（TCID50）。形态学和其他生物物理特征属于典型腺病毒：直径74nm的二十面体外壳、长度29nm的纤维突起、在受感染肾细胞内的定位、在氯化铯中的浮力密度为1.34g/ml、DNA合成抑制剂对其增殖的抑制作用。其热稳定性高于人腺病毒，而与此相反，MAV在很大程度上会被胰蛋白酶灭活。未检测到完全或不完全的血凝素或毒素样活性。该病毒与人腺病毒无交叉中和反应，与另一株小鼠腺病毒K-87有单向交叉反应。MAV的可溶性补体结合抗原的沉降率为12S，与人腺病毒的六邻体成分相同；在与适当抗血清的试验中，两种抗原显示出部分抗原关系。
