Sanjuan N A, Lascano E F
Arch Virol. 1986;91(3-4):329-39. doi: 10.1007/BF01314291.
Peroxidase-antiperoxidase technique and histology were employed to elucidate the peripheral routes involved in HSV-2 progression from vagina towards the central nervous system in mice. 12 week-old female Balb/c mice were intravaginally infected with 5 X 10(5)LD50 of HSV-2. Sixty per cent of animals developed vulvovaginitis, perigenital alopecia and hind-limb paresia. Death occurred at 9-11 days post-infection. Colon dilatation and urinary bladder distention were observed in all cases. Complete transversal sections from vulva to kidneys were obtained of each animal, including the spinal cord in situ. Herpes antigen were regularly detected in vulvovaginal epithelium, intramural, perigenital and perivesical small nerves. Besides, their invariable presence in Auerbach's plexus and sympathetic ganglia, strongly suggests preferential autonomic nervous system involvement in the progression of HSV-2 intravaginal infection towards the spinal cord.
采用过氧化物酶-抗过氧化物酶技术和组织学方法，以阐明小鼠中单纯疱疹病毒2型（HSV-2）从阴道向中枢神经系统进展所涉及的外周途径。12周龄雌性Balb/c小鼠经阴道感染5×10⁵半数致死剂量（LD50）的HSV-2。60%的动物出现外阴阴道炎、生殖器周围脱毛和后肢轻瘫。感染后9 - 11天死亡。所有病例均观察到结肠扩张和膀胱膨胀。对每只动物获取从外阴到肾脏的完整横切面，包括原位脊髓。在外阴阴道上皮、壁内、生殖器周围和膀胱周围小神经中经常检测到疱疹抗原。此外，它们在奥尔巴赫神经丛和交感神经节中始终存在，强烈提示自主神经系统在HSV-2阴道内感染向脊髓进展过程中优先受累。
