普朗丁通过阻断钙通道松弛分离的大鼠主动脉环。

Prunetin Relaxed Isolated Rat Aortic Rings by Blocking Calcium Channels.

机构信息

Department of Herbology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.

Department of Herbology, Graduate School, Kyung Hee University, Seoul 02447, Korea.

出版信息

Molecules. 2018 Sep 17;23(9):2372. doi: 10.3390/molecules23092372.

Prunetin, a component of herbal medicines and various foods, such as pea, peach, cherry, and , is a useful pharmacological compound. We previously reported the potent vasorelaxant effect of the bark of . Therefore, we investigated the vasorelaxant activities of prunetin on isolated rat aortic rings and hypotensive activity on spontaneously hypertensive rats (SHR) in this study. In the present study, prunetin (1⁻30 μg/mL) relaxed isolated rat aortic rings pre-contracted by phenylephrine (PE) in a concentration-dependent manner. Pre-incubation with prunetin (3 and 10 μg/mL) inhibited vasoconstriction induced by the supply of Ca in rat aortic rings pre-contracted with PE or KCl in a Ca-free Krebs⁻Henseleit (KH) buffer. Prunetin (10 μg/mL) pre-treatment also inhibited caffeine-induced contraction of aortic rings in a Ca-free KH buffer. To investigate the hypotensive effect of prunetin, the systolic blood pressure (SBP) of the SHR was measured by using a tail cuff assay. The SBP of SHR was significantly lower in the prunetin (25 mg/kg)-treated group. These results suggested that prunetin decreased blood pressure and relaxed blood vessels by blocking receptor-operated calcium channels, voltage-dependent calcium channels, and ryanodine receptor channels.

普那亭是草药和各种食物（如豌豆、桃、樱桃等）的成分，是一种有用的药理学化合物。我们之前报道过朴树皮具有很强的血管舒张作用。因此，在这项研究中，我们研究了普那亭对离体大鼠主动脉环的血管舒张活性和对自发性高血压大鼠（SHR）的降压活性。在本研究中，普那亭（1⁻30 μg/mL）以浓度依赖性方式松弛了由苯肾上腺素（PE）预收缩的离体大鼠主动脉环。普那亭（3 和 10 μg/mL）预孵育抑制了在 PE 或 KCl 预收缩的大鼠主动脉环中由 Ca 供应引起的血管收缩，或在不含 Ca 的 Krebs⁻Henseleit（KH）缓冲液中。普那亭（10 μg/mL）预处理也抑制了在不含 Ca 的 KH 缓冲液中咖啡因引起的主动脉环收缩。为了研究普那亭的降压作用，通过尾套法测量 SHR 的收缩压（SBP）。普那亭（25 mg/kg）处理组的 SHR 的 SBP 显著降低。这些结果表明，普那亭通过阻断受体操纵的钙通道、电压依赖性钙通道和ryanodine 受体通道来降低血压和舒张血管。