Figueiredo Eugênia Abrantes de, Alves Naiane Ferraz Bandeira, Monteiro Matheus Morais de Oliveira, Cavalcanti Clenia de Oliveira, Silva Tania Maria Sarmento da, Silva Telma Maria Guedes da, Braga Valdir de Andrade, Oliveira Eduardo de Jesus
Centro de Biotecnologia, Programa de Pós Graduação em Produtos Naturais e Sintéticos Bioativos, Campus I, João Pessoa, PB 58051-970, Brazil.
Departamento de Química, Laboratório de Bioprospecção Fitoquímica, Universidade Federal Rural de Pernambuco, Recife, PE 52171-900, Brazil.
Nutrients. 2017 Jun 3;9(6):574. doi: 10.3390/nu9060574.
A particularly phenolic-rich fraction extracted from red wine from the São Francisco valley (Northeastern Brazil) was chemically characterized and its hypotensive and antioxidant effects on spontaneously hypertensive rats were studied both in vitro and in vivo. The liquid-liquid pH dependent fractionation scheme afforded a fraction with high content of bioactive phenolics such as flavonols, flavonol glycosides, phenolic acids and anthocyanins, whose identities were confirmed by liquid chromatography coupled to mass spectrometry analysis. Pretreatment of spontaneously hypertensive rats with this wine fraction at doses of 50 and 100 mg/kg by gavage. for 15 days was able to decrease mean arterial pressure and heart rate as well as decrease serum lipid peroxidation. The fraction at concentrations of 0.01-1000 µg/mL induced concentration-dependent relaxation of isolated rat superior mesenteric artery rings pre-contracted with phenylephrine and this effect was not attenuated by endothelium removal. Our results demonstrate it is possible for phenolic constituents of red wine that are orally bioavailable to exert in vivo hypotensive and antioxidant effects on intact endothelial function.
从巴西东北部圣弗朗西斯科山谷的红葡萄酒中提取的一种富含酚类物质的特定组分进行了化学表征，并在体外和体内研究了其对自发性高血压大鼠的降压和抗氧化作用。液-液pH依赖性分级分离方案得到了一个富含生物活性酚类物质的组分，如黄酮醇、黄酮醇苷、酚酸和花青素，其成分通过液相色谱-质谱联用分析得以确认。将该葡萄酒组分以50和100 mg/kg的剂量通过灌胃给予自发性高血压大鼠，持续15天，能够降低平均动脉压和心率，并减少血清脂质过氧化。该组分在浓度为0.01 - 1000 µg/mL时，可诱导苯肾上腺素预收缩的离体大鼠肠系膜上动脉环产生浓度依赖性舒张，且这种作用不会因去除内皮而减弱。我们的结果表明，口服生物可利用的红葡萄酒酚类成分有可能对完整的内皮功能产生体内降压和抗氧化作用。
