Universidad Nacional Autónoma de México Unidad León, Río Blanco, Guanajuato, Mexico.
Universidad Autónoma de San Luis Potosí, Zona Universitaria, San Luis Potosí, Mexico.
Odontology. 2019 Apr;107(2):223-230. doi: 10.1007/s10266-018-0389-7. Epub 2018 Sep 18.
We explored chitosan-based sustained release pastes for apexification. The study aimed to formulate chitosan-based pastes loaded with calcium hydroxide (CH) or with calcium chloride (CC), and to evaluate the sustained release of Ca and pH changes in deionized water as well as the effect of the pastes on cell viability. The pastes were formulated by dissolution of the chitosan in 1% or 2% acetic acid (AAC) plus the addition of CH or CC, then were suspended in deionized water for 50 days; the released Ca(II) and pH were measured with an electrode probe. The effect of the pastes on viability of human dental pulp cells was evaluated with a MTS assay. The results showed that the pastes prepared with 1% and 2% AAC and loaded with CH released a 74.9% and a 76.1% of the Ca content, respectively, while the pastes prepared with 1% and 2% AAC loaded with CC released a content of Ca of 90.8% and 76.6%, respectively. A control paste (CH and polyethylene glycol) released a 95.4%; significant statistical differences were found between the percentage of the experimental pastes and the control. The CH-loaded pastes caused an alkaline pH at the starting of the study, but the pH became neutral at the ending. The pH of the CC-loaded pastes was neutral at the starting and was acid at the ending. The pastes no affected on the cell viability. The chitosan-based pastes showed a suitable sustained release profile and cytocompatibility.
我们探索了壳聚糖基的控释糊剂用于根尖诱导成形术。本研究旨在制备负载氢氧化钙（CH）或氯化钙（CC）的壳聚糖糊剂，并评估其在去离子水中的 Ca 持续释放和 pH 变化，以及糊剂对细胞活力的影响。糊剂通过在 1%或 2%醋酸（AAC）中溶解壳聚糖并加入 CH 或 CC 来制备，然后悬浮在去离子水中 50 天；用电极探针测量释放的 Ca(II)和 pH。通过 MTS 测定评估糊剂对人牙髓细胞活力的影响。结果表明，用 1%和 2% AAC 制备并负载 CH 的糊剂分别释放了 74.9%和 76.1%的 Ca 含量，而用 1%和 2% AAC 制备并负载 CC 的糊剂分别释放了 90.8%和 76.6%的 Ca 含量。一个对照糊剂（CH 和聚乙二醇）释放了 95.4%；实验糊剂和对照糊剂之间的百分比有显著的统计学差异。负载 CH 的糊剂在研究开始时呈碱性 pH，但在结束时呈中性 pH。负载 CC 的糊剂在开始时呈中性 pH，在结束时呈酸性 pH。糊剂对细胞活力没有影响。壳聚糖基糊剂显示出合适的持续释放特性和细胞相容性。
