Takeya K, Shimotori S, Taniguchi T, Nomoto K
J Gen Microbiol. 1977 Jun;100(2):373-9. doi: 10.1099/00221287-100-2-373.
Listeria monocytogenes, in doses of 2-0 X 10(3) to 3-0 X 10(3) viable organisms, was injected into athymic nude mice, irradiated mice and mice treated with reticuloendothelial system-blocking agents. Viable counts on liver and spleen homogenates were made at intervals after infection. In both nude mice (nu/nu) and normal littermates (nu/+) of BALB/c background, the bacteria grew rapidly for 24 h but increased only slowly thereafter, to reach a plateau of about 10(5) per organ at 72 h. In nu/+ mice, the number of viable bacteria began to decrease after 6 to 9 days, with complete elimination by day 12. In nude mice, the number of Listeria remained at a stable level of approximately 10(5) per organ during the observation period of 21 days. In lethally irradiated nu/+ mice, bacteria grew progressively and extensively to reach 10(7) per spleen and 10(9) per liver by 72 h. Bacterial growth during the first 72 h was markedly enhanced by treatment with carbon particles, dextran sulphate 500 or silica. These enhancing effects were also observed in nude mice and in AKR, C3H/He and C57BL/6 animals. We conclude that both non-immune phagocytes and T cell-dependent mechanisms contribute to the resistance of mice to Listeria infection.
将剂量为2.0×10³至3.0×10³个活的单核细胞增生李斯特菌注射到无胸腺裸鼠、经辐射的小鼠以及用网状内皮系统阻断剂处理过的小鼠体内。感染后定期对肝脏和脾脏匀浆进行活菌计数。在BALB/c背景的无胸腺裸鼠（nu/nu）和正常同窝小鼠（nu/+）中，细菌在24小时内迅速生长，但此后增长缓慢，至72小时时每个器官达到约10⁵的稳定水平。在nu/+小鼠中，活菌数量在6至9天后开始减少，到第12天时完全清除。在无胸腺裸鼠中，在21天的观察期内，李斯特菌数量在每个器官中保持在约10⁵的稳定水平。在接受致死剂量辐射的nu/+小鼠中，细菌逐渐大量生长，到72小时时每个脾脏达到10⁷，每个肝脏达到10⁹。用碳颗粒、硫酸葡聚糖500或二氧化硅处理可显著增强最初72小时内的细菌生长。在无胸腺裸鼠以及AKR、C3H/He和C57BL/6动物中也观察到了这些增强作用。我们得出结论，非免疫性吞噬细胞和T细胞依赖性机制均有助于小鼠对李斯特菌感染的抵抗力。
