CD32在HIV-1感染过程中的作用。
The role of CD32 during HIV-1 infection.
作者信息
Bertagnolli Lynn N, White Jennifer A, Simonetti Francesco R, Beg Subul A, Lai Jun, Tomescu Costin, Murray Alexandra J, Antar Annukka A R, Zhang Hao, Margolick Joseph B, Hoh Rebecca, Deeks Stephen G, Tebas Pablo, Montaner Luis J, Siliciano Robert F, Laird Gregory M, Siliciano Janet D
机构信息
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Howard Hughes Medical Institute, Baltimore, MD, USA.
出版信息
Nature. 2018 Sep;561(7723):E17-E19. doi: 10.1038/s41586-018-0494-3. Epub 2018 Sep 19.
Persistence of latent HIV-1 in long-lived resting memory CD4+ T cells is a major barrier to curing HIV-1 infection, and thus a biomarker for latently infected cells would be of great scientific and clinical importance. Through an elegant discovery-based approach, Descours . reported that CD32a, an Fcγ receptor not normally expressed on T cells, is a potential biomarker for latently infected cells. Using the quantitative viral outgrowth assay, we show that CD32+ CD4+ T cells do not harbor the majority of intact proviruses in the latent reservoir and that the enrichment found by Descours . may in part reflect the use of an ultrasensitive ELISA for HIV-1 p24 antigen that does not predict exponential viral outgrowth. Our studies show that CD32 is not a biomarker for the major population of latently infected CD4+ T cells.
潜伏的HIV-1在长寿的静止记忆CD4+ T细胞中的持续存在是治愈HIV-1感染的主要障碍，因此，潜伏感染细胞的生物标志物具有重大的科学和临床意义。通过一种基于发现的精妙方法，德斯科尔等人报告称，CD32a是一种通常不在T细胞上表达的Fcγ受体，是潜伏感染细胞的潜在生物标志物。使用定量病毒增殖试验，我们发现CD32+ CD4+ T细胞并不包含潜伏库中大部分完整的前病毒，德斯科尔等人发现的富集现象可能部分反映了使用了一种超灵敏的HIV-1 p24抗原ELISA，该方法无法预测病毒的指数增殖。我们的研究表明，CD32不是潜伏感染的CD4+ T细胞主要群体的生物标志物。
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