斯里兰卡一家医院产碳青霉烯酶菌株中可移动毒力因子和多药耐药基因的特征分析
Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing in a Sri Lankan Hospital.
作者信息
Zhu Chendi, Liyanapathirana Veranja, Li Carmen, Pinto Vasanthi, Hui Mamie, Lo Norman, Wong Kam T, Dissanayake Nilanthi, Ip Margaret
机构信息
Department of Microbiology, The Chinese University of Hong Kong, Shatin, Hong Kong.
Department of Microbiology, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka.
出版信息
Front Microbiol. 2018 Aug 31;9:2044. doi: 10.3389/fmicb.2018.02044. eCollection 2018.
Limited data is available on the epidemiology and characteristics of carbapenem-resistant (CRE) and their associated plasmids or virulence determinants from Sri Lanka. Through whole genome sequencing of CREs from the intensive care units of a Sri Lankan teaching hospital, we identified a carbapenemase gene, in 10 carbapenemase-producing isolates (two strains of ST437 and eight strains of ST147) from 379 respiratory specimens. was carried in three variants of ColE-type plasmids. strains with variants showed high minimum inhibitory concentrations to carbapenem. Furthermore, genes encoding for extended spectrum β-lactamases (ESBL), plasmid-mediated quinolone resistance (PMQR) determinants (, , and ) were present in all 10 strains. Amino acid substitution in chromosomal quinolone resistance-determining regions (QRDRs) (Ser83Ile) and (Ser80Ile) were also observed. All strains had yersiniabactin genes on mobile element ICE. Strict infection control practices and judicious use of antibiotics are warranted to prevent further spread of multidrug-resistant .
关于斯里兰卡耐碳青霉烯类肠杆菌（CRE）的流行病学、特征及其相关质粒或毒力决定因素的数据有限。通过对斯里兰卡一家教学医院重症监护病房的CRE进行全基因组测序，我们从379份呼吸道标本中，在10株产碳青霉烯酶分离株（两株ST437和八株ST147）中鉴定出一个碳青霉烯酶基因。该基因存在于ColE型质粒的三种变体中。携带这些变体的菌株对碳青霉烯类药物表现出高最低抑菌浓度。此外，所有10株菌株中均存在编码超广谱β-内酰胺酶（ESBL）、质粒介导喹诺酮耐药（PMQR）决定因素（qnrA、qnrB和qnrS）的基因。还观察到染色体喹诺酮耐药决定区域（QRDRs）gyrA（Ser83Ile）和parC（Ser80Ile）的氨基酸替换。所有菌株在移动元件ICE上都有耶尔森菌素基因。必须采取严格的感染控制措施并合理使用抗生素，以防止多重耐药性CRE的进一步传播。
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