Xue Rui, Feng Jinsong, Ma Lina, Liu Chunrong, Xian Ming, Konkel Michael E, Wang Shuo, Lu Xiaonan
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, China.
Food, Nutrition, and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, Canada.
Front Microbiol. 2018 Aug 31;9:2074. doi: 10.3389/fmicb.2018.02074. eCollection 2018.
Two metal oxide (i.e., AlO and TiO) nanoparticles and ajoene, a garlic-derived organosulfur compound, were identified to be effective antimicrobials against , a leading cause of human gastrointestinal diseases worldwide. A significant synergistic antimicrobial effect was observed using ajoene and AlO/TiO nanoparticles in a combined manner to cause at least 8 log CFU/mL reduction of cells. Whole transcriptome sequencing (RNA-seq) and confocal micro-Raman spectroscopic analyses revealed the antimicrobial mechanism and identified the roles of ajoene and metal oxide nanoparticles in the synergistic treatment. Ajoene and metal oxide nanoparticles mediated a two-phase antimicrobial mechanism. Ajoene served as the inducing factor at the first phase that caused injury of cell membranes and increased the susceptibility of to stress. Metal oxide nanoparticles served as the active factor at the second phase that targeted sensitive cells and physically disrupted cell structure. This synergistic antimicrobial treatment demonstrates a potential to reduce the prevalence of and other pathogens on food contact surfaces and in the food chain.
两种金属氧化物（即氧化铝和二氧化钛）纳米颗粒以及大蒜衍生的有机硫化合物大蒜素，被确定为对全球人类胃肠道疾病的主要致病菌具有有效的抗菌作用。观察到大蒜素与氧化铝/二氧化钛纳米颗粒联合使用具有显著的协同抗菌效果，可使细胞减少至少8个对数CFU/mL。全转录组测序（RNA-seq）和共聚焦显微拉曼光谱分析揭示了抗菌机制，并确定了大蒜素和金属氧化物纳米颗粒在协同治疗中的作用。大蒜素和金属氧化物纳米颗粒介导了一种两阶段抗菌机制。大蒜素在第一阶段作为诱导因子，导致细胞膜损伤并增加细胞对压力的敏感性。金属氧化物纳米颗粒在第二阶段作为活性因子，靶向敏感细胞并物理破坏细胞结构。这种协同抗菌处理显示出降低食品接触表面和食物链中该致病菌及其他病原体流行率的潜力。
