a Department of Life Sciences , University of Modena and Reggio Emilia , Reggio Emilia , Italy.
Int J Food Sci Nutr. 2019 May;70(3):335-348. doi: 10.1080/09637486.2018.1513996. Epub 2018 Sep 20.
In this study, the bioaccessibility of phenolic compounds after in vitro gastrointestinal digestion of two cherry cultivars was assessed. The phenolic profile was modified during in vitro digestion, with a considerable decrease of total and individual phenolic compounds. Hydroxycinnamic acids and especially coumaroylquinic acids showed the highest bioaccessibility. Isomerisation of caffeoylquinic and coumaroylquinic acids was observed after in vitro digestion. Modification of the phenolic profile after digestion resulted in an increased or decreased scavenging activity depending on the assay. In vitro digested phenolic-rich fractions also showed antiproliferative activity against SW480 but no effect against Caco-2 cell lines. Both Caco-2 and SW480 cell lines were able to metabolise cherry phenolic compounds with remarkable differences. An accumulation of glycosylated flavonols was observed in SW480 medium. In conclusion, phenolic compounds from cherries and especially hydroxycinnamic acids were efficiently released and remained bioaccessible after in vitro digestion, resulting in antioxidant and antiproliferative activities.
本研究评估了两种樱桃品种经体外胃肠道消化后酚类化合物的生物可给性。酚类谱在体外消化过程中发生了改变，总酚类化合物和各单体酚类化合物的含量均显著降低。羟基肉桂酸和尤以咖啡酰奎尼酸具有最高的生物可给性。体外消化后观察到咖啡酰奎尼酸和香豆酰奎尼酸的异构化。消化后酚类谱的改变导致清除活性的增加或降低，具体取决于检测方法。体外消化的富含酚类的馏分也对 SW480 具有抗增殖活性，但对 Caco-2 细胞系没有影响。Caco-2 和 SW480 细胞系均能够代谢樱桃酚类化合物，但存在显著差异。在 SW480 培养基中观察到糖苷化类黄酮的积累。总之，樱桃中的酚类化合物，尤其是羟基肉桂酸，在体外消化后能够有效释放并保持生物可给性，从而具有抗氧化和抗增殖活性。
