Hernández José Ángel, Cazapal-Monteiro Cristiana Filipa, Sanchís Jaime, Sánchez-Andrade Rita, Paz-Silva Adolfo, Arias María Sol
1 Control of Parasites Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela , Lugo, Spain .
2 Parasitología y Enfermedades Parasitarias, Universidad de La República , Salto, Uruguay .
Vector Borne Zoonotic Dis. 2018 Dec;18(12):690-696. doi: 10.1089/vbz.2018.2296. Epub 2018 Sep 19.
The usefulness of a strategy involving the biological control of soil-transmitted helminths (STHs) with zoonotic potential is tested. A blend of spores of two filamentous fungi, Mucor circinelloides and Duddingtonia flagrans, was provided to one group (G-2) of puppies (five Griffon bleu de Gascogne and five Gascon Saintongeois) shedding eggs of Toxocara canis, Toxascaris leonina, Trichuris vulpis, and Ancylostoma caninum. Other identical group (G-1) remained without spores. Fecal samples were collected daily and divided into two portions, one sample was immediately analyzed by the flotation test and the other was examined 28 days later. Viability of eggs of T. canis, T. leonina, and T. vulpis in fecal pats was ascertained by microscopic examination; the numbers of larvae of A. caninum were assessed in each group. In the feces of G-2, spores of both fungi were observed 1 day after beginning the assay. Viability of eggs of T. canis was reduced by 57%, T. leonina by 61% and T. vulpis by 53% in the fecal pats of G-2, and by 87%, 92%, and 85%, respectively, in G-1. The numbers of third-stage larvae of A. caninum were 59% lower in G-2 than that in G-1. By considering the results obtained and the absence of adverse effects in the puppies of G-2, it is concluded that the presence of the filamentous fungi in the feces has a high potential to avoid the transmission of these parasites. Further studies are in progress to determine the appropriate way of administration to pets.
对一种涉及利用有潜在人畜共患病原体的土壤传播蠕虫（STH）进行生物防治的策略的有效性进行了测试。将两种丝状真菌——卷枝毛霉和达丁顿霉的孢子混合物提供给一组（G-2）排出犬弓首蛔虫、狮弓蛔虫、狐毛首线虫和犬钩虫虫卵的幼犬（五只加斯科涅蓝犬和五只加斯科涅圣东日犬）。另一组相同的幼犬（G-1）不接触孢子。每天收集粪便样本并分成两份，一份样本立即通过浮选试验进行分析，另一份样本在28天后进行检查。通过显微镜检查确定粪便中犬弓首蛔虫、狮弓蛔虫和狐毛首线虫虫卵的活力；评估每组中犬钩虫幼虫的数量。在G-2组的粪便中，在试验开始1天后观察到两种真菌的孢子。在G-2组的粪便中，犬弓首蛔虫虫卵的活力降低了57%，狮弓蛔虫虫卵降低了61%，狐毛首线虫虫卵降低了53%；在G-1组中，分别降低了87%、92%和85%。G-2组中犬钩虫第三期幼虫的数量比G-1组低59%。考虑到所获得的结果以及G-2组幼犬未出现不良反应，得出结论：粪便中丝状真菌的存在具有很高的潜力来避免这些寄生虫的传播。目前正在进行进一步的研究，以确定对宠物的合适给药方式。
