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通过血清中和试验和DNA限制性内切酶分析对41株19/37型腺病毒进行进一步鉴定。

Further characterization of 41 isolates of adenovirus types 19/37 by serum neutralization and DNA restriction enzyme analysis.

作者信息

Meng Z D, Kennett M L, Rodger S M, Dickson K E, Anderson B N, Gust I D

出版信息

J Hyg (Lond). 1986 Oct;97(2):377-83. doi: 10.1017/s0022172400065463.

DOI:10.1017/s0022172400065463
PMID:3023482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2083553/
Abstract

Forty-one strains of adenovirus type 19/37 (Ad19/37) mainly isolated from patients with keratoconjunctivitis or conjunctivitis between 1974 and 1984 were re-evaluated by serum neutralization (SN), haemagglutination inhibition (HI) and DNA restriction analysis. Of 19 isolates which were neutralized to high titre by antiserum prepared against prototype Ad19, 5 showed cross-reactivity with 32-64 units of Ad37 antiserum, while of 22 strains neutralized by high titre by Ad37 antiserum, 3 showed cross-reactivity with 32 units of Ad19 antiserum. By DNA restriction analysis, all Ad19 isolates were identical to each other and to Ad19A virus. Using endonuclease Bgl 1, three variants were observed among the Ad37 isolates.

摘要

对1974年至1984年间主要从角结膜炎或结膜炎患者中分离出的41株19/37型腺病毒(Ad19/37),通过血清中和试验(SN)、血凝抑制试验(HI)和DNA限制性分析进行了重新评估。在19株被针对原型Ad19制备的抗血清高滴度中和的分离株中,5株与32 - 64单位的Ad37抗血清表现出交叉反应,而在22株被Ad37抗血清高滴度中和的菌株中,3株与32单位的Ad19抗血清表现出交叉反应。通过DNA限制性分析,所有Ad19分离株彼此相同,且与Ad19A病毒相同。使用内切酶Bgl 1,在Ad37分离株中观察到三个变体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba7/2083553/0568584b9e4c/jhyg00002-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba7/2083553/0568584b9e4c/jhyg00002-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba7/2083553/0568584b9e4c/jhyg00002-0183-a.jpg

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A rapid and simple method for preparation of adenovirus DNA from infected cells.一种从感染细胞中制备腺病毒DNA的快速简便方法。
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The changing etiology of epidemic keratoconjunctivitis: antigenic and restriction enzyme analyses of adenovirus types 19 and 37 isolated over a 10-year period.
流行性角结膜炎不断变化的病因:对10年间分离出的19型和37型腺病毒的抗原及限制酶分析
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Keratoconjunctivitis associated with adenovirus type 37: an extended outbreak in an ophthalmologist's office.与37型腺病毒相关的角结膜炎:眼科医生办公室内的一次大规模暴发
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Characterization of candidate adenovirus 37 by SDS-polyacrylamide gel electrophoresis of virion polypeptides and DNA restriction site mapping.通过病毒粒子多肽的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和DNA限制酶切位点图谱分析对37型腺病毒候选株进行鉴定。
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