Heath R, Birch C, Gust I
J Gen Virol. 1986 Nov;67 ( Pt 11):2455-66. doi: 10.1099/0022-1317-67-11-2455.
Five neutralizing monoclonal antibodies produced against human rotavirus (HRV) serotypes 1, 2, 3 and 4 and the simian rotavirus (SA11) were used to study 59 rotavirus isolates of human, simian and feline origin previously serotyped using polyclonal antisera. In neutralization tests, 19 of 26 HRV serotype 1 isolates, both strains of HRV serotype 2, 14 of 24 HRV serotype 3 isolates and all of seven serotype 4 isolates were neutralized by the homologous serotype-specific monoclonal antibodies. Use of the panel of monoclonal antibodies revealed antigenic differences between strains within serotypes 1 and 3 and, in the case of the serotype 3 strains, each variant had a unique RNA electropherotype. An enzyme immunoassay (EIA) which utilized the monoclonal antibodies essentially confirmed the neutralization results. Preliminary results show that direct serotyping in faecal extracts by EIA using these monoclonal antibodies is specific but lacks sensitivity.
针对人轮状病毒（HRV）血清型1、2、3和4以及猿猴轮状病毒（SA11）产生的5种中和性单克隆抗体，被用于研究59株先前使用多克隆抗血清进行血清分型的人、猿猴和猫源轮状病毒分离株。在中和试验中，26株HRV血清型1分离株中的19株、HRV血清型2的两个毒株、24株HRV血清型3分离株中的14株以及所有7株血清型4分离株均被同源血清型特异性单克隆抗体中和。使用该单克隆抗体组合揭示了血清型1和3内各毒株之间的抗原差异，并且就血清型3毒株而言，每个变体都有独特的RNA电泳图谱。一种利用这些单克隆抗体的酶免疫测定（EIA）基本上证实了中和结果。初步结果表明，使用这些单克隆抗体通过EIA对粪便提取物进行直接血清分型具有特异性，但缺乏敏感性。
