Brüssow H, Eichhorn W, Rohwedder A, Snodgrass D, Sidoti J
Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, Lausanne, Switzerland.
J Gen Virol. 1991 Jul;72 ( Pt 7):1559-67. doi: 10.1099/0022-1317-72-7-1559.
Neutralizing antibodies against 10 serotypes of rotavirus were measured in sera from different age groups of German cattle. Only five of 143 sera did not neutralize heterologous serotypes. Sera from 64 of 76 calves younger than 1 year neutralized bovine rotavirus NCDV (serotype 6). From these calves, sera 54, 26, 51, 24, 12, 10 and 37, in neutralized addition, the heterologous serotypes 1, 2, 3, 4, 5, 7 and 9, respectively. Thirty-eight of 46 rotavirus isolates from Bavarian calves with diarrhoea were serotyped by neutralization: 22, 2 and 14 isolates were typed as serotype 6, serotype 10 (B223) and a newly defined subtype of serotype 10 (V1005), respectively. All serotype 6 isolates and none of the serotype 10 or V1005-like viruses tested hybridized to a NCDV-specific cDNA probe. Eight isolates gave equivocal results by neutralization. We failed however to identify serotype 1, 2, 3, 4 or 8 bovine rotavirus isolates by neutralization with hyperimmune sera and dot blot hybridization with serotype-specific cDNA probes. Thus cross-reacting antibodies in cattle might not represent an anamnestic response, but the recognition of a cross-reacting neutralization epitope shared by many rotavirus serotypes.
检测了德国不同年龄组牛血清中针对10种轮状病毒血清型的中和抗体。143份血清中只有5份不能中和异源血清型。76头1岁以下犊牛的血清中有64份能中和牛轮状病毒NCDV（血清型6）。在这些犊牛中，血清54、26、51、24、12、10和37分别还能中和异源血清型1、2、3、4、5、7和9。对来自巴伐利亚腹泻犊牛的46份轮状病毒分离株进行了中和血清分型：22份、2份和14份分离株分别被鉴定为血清型6、血清型10（B223）和一种新定义的血清型10亚型（V1005）。所有检测的血清型6分离株与NCDV特异性cDNA探针杂交，而血清型10或V1005样病毒均未杂交。8份分离株通过中和试验得到了模棱两可的结果。然而，我们未能通过用超免疫血清中和及用血清型特异性cDNA探针进行斑点杂交来鉴定血清型1、2、3、4或8的牛轮状病毒分离株。因此，牛体内的交叉反应抗体可能并不代表回忆反应，而是对许多轮状病毒血清型共有的交叉反应中和表位的识别。
