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通过与源自编码外衣壳蛋白VP7的基因高变区的聚合酶链反应生成的探针杂交来鉴定人轮状病毒血清型。

Identification of human rotavirus serotype by hybridization to polymerase chain reaction-generated probes derived from a hyperdivergent region of the gene encoding outer capsid protein VP7.

作者信息

Flores J, Sears J, Schael I P, White L, Garcia D, Lanata C, Kapikian A Z

机构信息

Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

J Virol. 1990 Aug;64(8):4021-4. doi: 10.1128/JVI.64.8.4021-4024.1990.

DOI:10.1128/JVI.64.8.4021-4024.1990
PMID:2164610
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249704/
Abstract

We have synthesized 32P-labeled hybridization probes from a hyperdivergent region (nucleotides 51 to 392) of the rotavirus gene encoding the VP7 glycoprotein by using the polymerase chain reaction method. Both RNA (after an initial reverse transcription step) and cloned cDNA from human rotavirus serotypes 1 through 4 could be used as templates to amplify this region. High-stringency hybridization of each of the four probes to rotavirus RNAs dotted on nylon membranes allowed the specific detection of corresponding sequences and thus permitted identification of the serotype of the strains dotted. The procedure was useful when applied to rotaviruses isolated from field studies.

摘要

我们通过聚合酶链反应方法，从编码VP7糖蛋白的轮状病毒基因的一个高度发散区域（核苷酸51至392）合成了32P标记的杂交探针。RNA（经过初始逆转录步骤后）和来自1至4型人轮状病毒血清型的克隆cDNA均可用作模板来扩增该区域。四种探针中的每一种与点样在尼龙膜上的轮状病毒RNA进行高严格度杂交，能够特异性检测相应序列，从而鉴定点样菌株的血清型。该方法应用于从现场研究中分离出的轮状病毒时很有用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/170c/249704/5858bb76faae/jvirol00063-0471-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/170c/249704/e1b3b27b3475/jvirol00063-0469-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/170c/249704/e23477eb29df/jvirol00063-0470-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/170c/249704/c91060b90253/jvirol00063-0470-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/170c/249704/05bc1c807671/jvirol00063-0471-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/170c/249704/5858bb76faae/jvirol00063-0471-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/170c/249704/e1b3b27b3475/jvirol00063-0469-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/170c/249704/e23477eb29df/jvirol00063-0470-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/170c/249704/c91060b90253/jvirol00063-0470-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/170c/249704/05bc1c807671/jvirol00063-0471-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/170c/249704/5858bb76faae/jvirol00063-0471-b.jpg

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Genetic relatedness among human rotavirus genes coding for VP7, a major neutralization protein, and its application to serotype identification.编码主要中和蛋白VP7的人轮状病毒基因之间的遗传相关性及其在血清型鉴定中的应用。
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