Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, ROC.
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, ROC; Food Industry Research and Development Institute, Hsinchu, Taiwan, ROC.
Food Chem. 2019 Jan 15;271:505-515. doi: 10.1016/j.foodchem.2018.07.064. Epub 2018 Aug 4.
Ara h2 is a major peanut allergen that induces rashes, vomiting, diarrhea, and anaphylactic shock. Since peanut is a major source in producing edible oils globally, Ara h2 residues can be present in various edible oils. In this work, an immunomagnetic nanoparticle-based lateral flow assay for identifying Ara h2 in edible oils is developed. This assay exhibits high sensitivity with a visual detection limit of 0.1 mg/kg Ara h2 in oil, and favorable specificity in differentiating peanut from seeds and nuts. The calculated CV values of intra- and inter-assay were 6.73-10.21% and 4.75-8.57%, respectively, indicating high reproducibility. In an analysis of 26 oil products, Ara h2 was detected in two peanut oils as 0.122 ± 0.026 mg/kg and 0.247 ± 0.027 mg/kg. The entire method takes 5 h, including a 3.5-h sample preparation. Hence, this method has the potential to be an effective way to screen edible oils for Ara h2.
Ara h2 是一种主要的花生过敏原，会引起皮疹、呕吐、腹泻和过敏性休克。由于花生是全球食用油的主要来源，因此 Ara h2 残留可能存在于各种食用油中。在这项工作中，开发了一种基于免疫磁性纳米颗粒的侧向流动分析方法，用于鉴定食用油中的 Ara h2。该测定法具有高灵敏度，在油中 Ara h2 的目视检测限为 0.1mg/kg，在区分花生与种子和坚果方面具有良好的特异性。内和间测定的计算 CV 值分别为 6.73-10.21%和 4.75-8.57%，表明重现性高。在对 26 种油产品的分析中，在两种花生油中检测到 Ara h2，含量分别为 0.122±0.026mg/kg 和 0.247±0.027mg/kg。整个方法需要 5 小时，包括 3.5 小时的样品制备。因此，该方法有可能成为筛选食用油中 Ara h2 的有效方法。
