Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, RJ 21941-598, Brazil.
Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, RJ 21941-909, Brazil.
Biosensors (Basel). 2020 Nov 29;10(12):194. doi: 10.3390/bios10120194.
Several individuals will experience accidental exposure to an allergen. In this sense, the industry has invested in the processes of removing allergenic compounds in food. However, accidental exposure to allergenic proteins can result from allergenic substances not specified on labels. Analysis of allergenic foods is involved in methods based on immunological, genetic, and mass spectrometry. The traditional methods have some limitations, such as high cost. In recent years, biosensor and nanoparticles combined have emerged as sensitive, selective, low-cost, and time-consuming techniques that can replace classic techniques. Nevertheless, each nanomaterial has shown a different potential to specific allergens or classes. This review used Preferred Reporting Items for Systematic Reviews and the Meta-Analysis guidelines (PRISMA) to approach these issues. A total of 104 articles were retrieved from a standardized search on three databases (PubMed, Scopus and Web of Science). The systematic review article is organized by the category of allergen detection and nanoparticle detection. This review addresses the relevant biosensors and nanoparticles as gold, carbon, graphene, quantum dots to allergen protein detection. Among the selected articles it was possible to notice a greater potential application on the allergic proteins Ah, in peanuts and gold nanoparticle-base as a biosensor. We envision that in our review, the association between biosensor and nanoparticles has shown promise in the analysis of allergenic proteins present in different food samples.
一些人会意外接触过敏原。从这个意义上说，食品行业已经投入资金用于去除食物中的过敏原化合物。然而，对过敏原蛋白的意外接触可能是由标签上未注明的过敏原物质引起的。过敏原食品的分析涉及基于免疫学、遗传学和质谱的方法。传统方法存在一些局限性，例如成本高。近年来，生物传感器和纳米粒子的结合已经成为敏感、选择性、低成本和耗时的技术，可以替代经典技术。然而，每种纳米材料对特定过敏原或类别显示出不同的潜力。本文综述使用系统评价和荟萃分析指南（PRISMA）来探讨这些问题。从三个数据库（PubMed、Scopus 和 Web of Science）进行标准化搜索，共检索到 104 篇文章。系统评价文章按过敏原检测和纳米粒子检测类别进行组织。本文综述了与过敏原蛋白检测相关的生物传感器和纳米粒子，如金、碳、石墨烯、量子点。在所选择的文章中，花生和金纳米粒子基生物传感器对 Ah 等过敏蛋白的潜在应用较多。我们设想，在我们的综述中，生物传感器和纳米粒子的结合在分析不同食物样本中的过敏原蛋白方面显示出了很大的潜力。
