利用磁性纳米颗粒的免疫层析测定法检测加工食品中的主要花生过敏原 Ara h 1。

An immunochromatographic assay utilizing magnetic nanoparticles to detect major peanut allergen Ara h 1 in processed foods.

机构信息

Food and Animal Product Safety Inspection Center, National Chung Hsing University, Taichung, Taiwan, ROC; Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, R.O.C.

Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, R.O.C.

出版信息

Food Chem. 2022 May 1;375:131844. doi: 10.1016/j.foodchem.2021.131844. Epub 2021 Dec 12.

DOI:10.1016/j.foodchem.2021.131844

PMID:34952385

Abstract

This study describes an immunomagnetic nanoparticle (IMNP)-based lateral flow assay (LFA) for detecting the major peanut allergen Ara h 1. We developed a clearly specific method in identifying peanut from ten other seeds and nuts, and a good visual limit of detection (vLOD) of 0.01 μg/mL Ara h 1 in PBS. PBS that contains 1 M NaCl and 2% Tween 20 was determined to be the optimal extraction buffer for isolating Ara h 1 from cookie, milk and chocolate with vLOD values of 0.5 μg/g, 0.5 μg/mL, and 1 μg/g, respectively. Forty two processed foods were simultaneously analyzed using this method and an AOAC-approved ELISA kit. The specificity and sensitivity of this assay were thus determined to be 100 and 95%, respectively. This new IMNP-based LFA has potential as a rapid tool for screening processed foods for Ara h 1 residues.

摘要

本研究描述了一种基于免疫磁性纳米颗粒（IMNP）的侧向流动检测（LFA）方法，用于检测主要的花生过敏原 Ara h 1。我们开发了一种能够清晰特异性识别花生的方法，可区分十种其他种子和坚果，并且在 PBS 中对 Ara h 1 的良好视觉检测限（vLOD）为 0.01μg/mL。确定 PBS 中含有 1M NaCl 和 2%吐温 20 是从饼干、牛奶和巧克力中分离 Ara h 1 的最佳提取缓冲液，vLOD 值分别为 0.5μg/g、0.5μg/mL 和 1μg/g。使用该方法和 AOAC 批准的 ELISA 试剂盒同时分析了 42 种加工食品。因此，该测定方法的特异性和灵敏度分别为 100%和 95%。这种基于新型 IMNP 的 LFA 具有作为筛选加工食品中 Ara h 1 残留的快速工具的潜力。