Food and Animal Product Safety Inspection Center, National Chung Hsing University, Taichung, Taiwan, ROC; Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, R.O.C.
Food Chem. 2022 May 1;375:131844. doi: 10.1016/j.foodchem.2021.131844. Epub 2021 Dec 12.
This study describes an immunomagnetic nanoparticle (IMNP)-based lateral flow assay (LFA) for detecting the major peanut allergen Ara h 1. We developed a clearly specific method in identifying peanut from ten other seeds and nuts, and a good visual limit of detection (vLOD) of 0.01 μg/mL Ara h 1 in PBS. PBS that contains 1 M NaCl and 2% Tween 20 was determined to be the optimal extraction buffer for isolating Ara h 1 from cookie, milk and chocolate with vLOD values of 0.5 μg/g, 0.5 μg/mL, and 1 μg/g, respectively. Forty two processed foods were simultaneously analyzed using this method and an AOAC-approved ELISA kit. The specificity and sensitivity of this assay were thus determined to be 100 and 95%, respectively. This new IMNP-based LFA has potential as a rapid tool for screening processed foods for Ara h 1 residues.
本研究描述了一种基于免疫磁性纳米颗粒（IMNP）的侧向流动检测（LFA）方法，用于检测主要的花生过敏原 Ara h 1。我们开发了一种能够清晰特异性识别花生的方法，可区分十种其他种子和坚果，并且在 PBS 中对 Ara h 1 的良好视觉检测限（vLOD）为 0.01μg/mL。确定 PBS 中含有 1M NaCl 和 2%吐温 20 是从饼干、牛奶和巧克力中分离 Ara h 1 的最佳提取缓冲液，vLOD 值分别为 0.5μg/g、0.5μg/mL 和 1μg/g。使用该方法和 AOAC 批准的 ELISA 试剂盒同时分析了 42 种加工食品。因此，该测定方法的特异性和灵敏度分别为 100%和 95%。这种基于新型 IMNP 的 LFA 具有作为筛选加工食品中 Ara h 1 残留的快速工具的潜力。
