α因子信息素与酿酒酵母a细胞的结合：解离常数和结合位点数量
Binding of alpha-factor pheromone to Saccharomyces cerevisiae a cells: dissociation constant and number of binding sites.
作者信息
Jenness D D, Burkholder A C, Hartwell L H
出版信息
Mol Cell Biol. 1986 Jan;6(1):318-20. doi: 10.1128/mcb.6.1.318-320.1986.
Abstract
The number of alpha-factor binding sites on yeast MATa cells (8,000) and the equilibrium dissociation constant (6 X 10(-9) M) were determined from direct binding experiments. These values correct our previously reported estimates (D. D. Jennes, A. C. Burkholder, and L. H. Hartwell, Cell 35:521-529, 1983) that were based on indirect isotope dilution studies, and they lead to a revised rate constant for the association process (kon = 3 X 10(5) mol-1 s-1).
摘要
通过直接结合实验确定了酵母MATa细胞上α因子结合位点的数量（8000个）和平衡解离常数（6×10⁻⁹ M）。这些值修正了我们之前基于间接同位素稀释研究报告的估计值（D. D. 詹尼斯、A. C. 伯克霍尔德和L. H. 哈特韦尔，《细胞》35:521 - 529，1983年），并得出了缔合过程的修正速率常数（kon = 3×10⁵ mol⁻¹ s⁻¹）。
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