Deb S, DeLucia A L, Baur C P, Koff A, Tegtmeyer P
Mol Cell Biol. 1986 May;6(5):1663-70. doi: 10.1128/mcb.6.5.1663-1670.1986.
The simian virus 40 core origin of replication consists of nucleotides 5211 through 31. These 64 base pairs contain three functional domains with strict sequence requirements and two spacer regions with relaxed sequence specificity but precise positional constraints. The early domain extends for 10 contiguous base pairs between nucleotides 5211 and 5220. A 9-base pair spacer from sequences 5221 through 5229 separates the early domain from the 23-base pair central palindrome that directs the binding of T antigen. The late end of the core between nucleotides 12 and 31 also contains spacer and sequence-specific functions that are not yet completely mapped. We propose that the sequence-specific domains are interaction sites for viral and cellular proteins, determinants of DNA conformation, or both. The spacers would position these signals at required distances and rotations relative to one another.
猿猴病毒40核心复制起点由5211至31号核苷酸组成。这64个碱基对包含三个具有严格序列要求的功能域以及两个序列特异性较宽松但位置限制精确的间隔区。早期结构域在5211至5220号核苷酸之间延伸10个连续的碱基对。5221至5229号序列中的一个9碱基对间隔区将早期结构域与指导T抗原结合的23碱基对中央回文结构分隔开。核心结构的晚期末端在12至31号核苷酸之间也包含尚未完全定位的间隔区和序列特异性功能。我们认为，序列特异性结构域是病毒和细胞蛋白的相互作用位点、DNA构象的决定因素，或兼而有之。间隔区会将这些信号定位在彼此所需的距离和旋转角度上。
