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一种突变的疱疹病毒蛋白导致其他病毒蛋白的核定位受阻。

A mutant herpesvirus protein leads to a block in nuclear localization of other viral proteins.

作者信息

Knipe D M, Smith J L

出版信息

Mol Cell Biol. 1986 Jul;6(7):2371-81. doi: 10.1128/mcb.6.7.2371-2381.1986.

DOI:10.1128/mcb.6.7.2371-2381.1986
PMID:3023931
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367790/
Abstract

The herpes simplex virus mutants KOS1.1 ts756 and HFEM tsLB2 express temperature-sensitive ICP4 proteins that are not localized properly to the cell nucleus at the nonpermissive temperature. In these infected cells at the nonpermissive temperature, nuclear localization of at least two other viral proteins, ICP0 and ICP8, is impaired. Replacement of the mutated sequences in the ICP4 gene of tsLB2 restored proper nuclear localization of all of the proteins. The ICP0 and ICP8 proteins expressed in cells transfected with their individual genes were localized to the cell nucleus. Therefore, in infected cells, the mutant ICP4 gene product appears to be the primary defect which leads to the block in nuclear localization of the other proteins. One viral protein, ICP27, was not inhibited for nuclear localization in these cells. These data indicate that there are at least two pathways for nuclear localization of HSV proteins, one of which is inhibited by the mutant ICP4 protein. The mutant ICP4 protein may define a probe for one of the pathways of nuclear localization of proteins.

摘要

单纯疱疹病毒突变体KOS1.1 ts756和HFEM tsLB2表达温度敏感的ICP4蛋白，在非允许温度下这些蛋白不能正确定位于细胞核。在非允许温度下的这些受感染细胞中，至少另外两种病毒蛋白ICP0和ICP8的核定位受损。tsLB2的ICP4基因中突变序列的替换恢复了所有蛋白的正确核定位。用其各自基因转染的细胞中表达的ICP0和ICP8蛋白定位于细胞核。因此，在受感染细胞中，突变的ICP4基因产物似乎是导致其他蛋白核定位受阻的主要缺陷。一种病毒蛋白ICP27在这些细胞中的核定位未受抑制。这些数据表明，单纯疱疹病毒蛋白的核定位至少有两条途径，其中一条被突变的ICP4蛋白抑制。突变的ICP4蛋白可能为蛋白核定位途径之一定义了一种探针。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/e13603a8aa5c/molcellb00091-0101-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/420c245e96e7/molcellb00091-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/d32d914e7e02/molcellb00091-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/5e6c40fbcb4b/molcellb00091-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/68cb6d8e20e9/molcellb00091-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/fcd694028b0b/molcellb00091-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/649b4ebe80a4/molcellb00091-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/3cec2b0b95f3/molcellb00091-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/0d1f3d6f4881/molcellb00091-0100-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/6d3bae2517f9/molcellb00091-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/e13603a8aa5c/molcellb00091-0101-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/420c245e96e7/molcellb00091-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/d32d914e7e02/molcellb00091-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/5e6c40fbcb4b/molcellb00091-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/68cb6d8e20e9/molcellb00091-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/fcd694028b0b/molcellb00091-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/649b4ebe80a4/molcellb00091-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/3cec2b0b95f3/molcellb00091-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/0d1f3d6f4881/molcellb00091-0100-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/6d3bae2517f9/molcellb00091-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/184e/367790/e13603a8aa5c/molcellb00091-0101-b.jpg

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本文引用的文献

1
RELATIONSHIP BETWEEN THE ENVELOPE AND THE INFECTIVITY OF HERPES SIMPLEX VIRUS.单纯疱疹病毒包膜与感染性之间的关系
Proc Soc Exp Biol Med. 1964 Mar;115:814-6. doi: 10.3181/00379727-115-29045.
2
The morphology of herpes virus.疱疹病毒的形态学
Virology. 1960 Oct;12:204-22. doi: 10.1016/0042-6822(60)90195-1.
3
Characterization of two temperature-sensitive mutants of type 5 adenovirus with mutations in the 100,000-dalton protein gene.100,000道尔顿蛋白基因发生突变的5型腺病毒两个温度敏感突变体的特性分析
宿主 IFI16、PML 和 Daxx 蛋白限制单纯疱疹病毒 1 复制的机制。
J Virol. 2018 Apr 27;92(10). doi: 10.1128/JVI.00057-18. Print 2018 May 15.
4
Viral Ubiquitin Ligase Stimulates Selective Host MicroRNA Expression by Targeting ZEB Transcriptional Repressors.病毒泛素连接酶通过靶向ZEB转录抑制因子刺激宿主特异性微小RNA表达。
Viruses. 2017 Aug 7;9(8):210. doi: 10.3390/v9080210.
5
Barrier-to-Autointegration Factor 1 (BAF/BANF1) Promotes Association of the SETD1A Histone Methyltransferase with Herpes Simplex Virus Immediate-Early Gene Promoters.自身整合屏障因子1（BAF/BANF1）促进SETD1A组蛋白甲基转移酶与单纯疱疹病毒立即早期基因启动子的结合。
mBio. 2015 May 26;6(3):e00345-15. doi: 10.1128/mBio.00345-15.
6
Proteomic analysis of the herpes simplex virus 1 virion protein 16 transactivator protein in infected cells.单纯疱疹病毒1型病毒体蛋白16反式激活蛋白在感染细胞中的蛋白质组学分析。
Proteomics. 2015 Jun;15(12):1957-67. doi: 10.1002/pmic.201500020. Epub 2015 Apr 29.
7
Analysis of herpes simplex virion tegument ICP4 derived from infected cells and ICP4-expressing cells.分析来自感染细胞和表达 ICP4 细胞的单纯疱疹病毒粒子被膜 ICP4。
PLoS One. 2013 Aug 6;8(8):e70889. doi: 10.1371/journal.pone.0070889. Print 2013.
8
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J Virol. 2010 Oct;84(20):10802-11. doi: 10.1128/JVI.00063-10. Epub 2010 Aug 4.
9
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PLoS One. 2010 Jun 8;5(6):e10975. doi: 10.1371/journal.pone.0010975.
10
Oligomerization of ICP4 and rearrangement of heat shock proteins may be important for herpes simplex virus type 1 prereplicative site formation.ICP4的寡聚化和热休克蛋白的重排可能对单纯疱疹病毒1型复制前位点的形成很重要。
J Virol. 2008 Jul;82(13):6324-36. doi: 10.1128/JVI.00455-08. Epub 2008 Apr 23.
J Virol. 1981 Nov;40(2):491-500. doi: 10.1128/JVI.40.2.491-500.1981.
4
Distribution of proteins between nucleus and cytoplasm of Amoeba proteus.变形虫细胞核与细胞质之间蛋白质的分布
J Cell Biol. 1981 Mar;88(3):516-25. doi: 10.1083/jcb.88.3.516.
5
Complex of simian virus 40 large tumor antigen and 48,000-dalton host tumor antigen.猴病毒40大T抗原与48000道尔顿宿主肿瘤抗原的复合物。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):105-9. doi: 10.1073/pnas.78.1.105.
6
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.一种决定核质蛋白向细胞核迁移的多肽结构域。
Cell. 1982 Sep;30(2):449-58. doi: 10.1016/0092-8674(82)90242-2.
7
The intranuclear location of a herpes simplex virus DNA-binding protein is determined by the status of viral DNA replication.单纯疱疹病毒DNA结合蛋白的核内定位由病毒DNA复制状态决定。
Cell. 1984 Apr;36(4):857-68. doi: 10.1016/0092-8674(84)90035-7.
8
Herpes simplex virus and protein transport are associated with the cytoskeletal framework and the nuclear matrix in infected BSC-1 cells.
Virology. 1983 Sep;129(2):501-7. doi: 10.1016/0042-6822(83)90190-3.
9
Nuclear localization of herpesvirus proteins: potential role for the cellular framework.疱疹病毒蛋白的核定位：细胞骨架的潜在作用。
Mol Cell Biol. 1983 Mar;3(3):315-24. doi: 10.1128/mcb.3.3.315-324.1983.
10
Definition of a series of stages in the association of two herpesviral proteins with the cell nucleus.两种疱疹病毒蛋白与细胞核结合过程中一系列阶段的定义。
J Virol. 1982 Jul;43(1):314-24. doi: 10.1128/JVI.43.1.314-324.1982.