Percival-Smith A, Segall J
Mol Cell Biol. 1986 Jul;6(7):2443-51. doi: 10.1128/mcb.6.7.2443-2451.1986.
A differential hybridization screen of a genomic yeast DNA library previously identified 14 genes of Saccharomyces cerevisiae that are expressed preferentially during sporulation. Three of these sporulation-specific genes, SPS1, SPS2, and SPS3, have been shown to be closely linked. A mutational analysis has demonstrated that expression of the SPS1 gene, but not the SPS2 gene, is essential for the completion of sporulation. A diploid MATa/MAT alpha strain homozygous for a disruption of the SPS1 gene failed to form asci when subjected to sporulation conditions. The 3' end of the transcript encoded by the SPS1 gene was found to map only 185 base pairs from the 5' end of the SPS2 gene. The SPS1-SPS2 intergenic region was shown to contain all of the regulatory sequences necessary for the sporulation-specific activation of the SPS2 gene as assessed by expression of a translational SPS2-lacZ fusion gene present on a replicating, centromere-containing plasmid. The fusion gene was found to be expressed at the same time during sporulation as the chromosomal wild-type SPS2 gene.
先前对基因组酵母DNA文库进行的差异杂交筛选鉴定出了14个酿酒酵母基因，这些基因在孢子形成过程中优先表达。其中三个孢子形成特异性基因SPS1、SPS2和SPS3已被证明紧密连锁。突变分析表明，SPS1基因而非SPS2基因的表达对于孢子形成的完成至关重要。一个SPS1基因破坏纯合的二倍体MATa/MATα菌株在进行孢子形成条件处理时未能形成子囊。发现SPS1基因编码的转录本的3'端与SPS2基因的5'端仅相距185个碱基对。通过存在于含着丝粒的复制质粒上的翻译SPS2-lacZ融合基因的表达评估，SPS1-SPS2基因间区域被证明包含SPS2基因孢子形成特异性激活所需的所有调控序列。发现融合基因在孢子形成过程中与染色体野生型SPS2基因同时表达。
