School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.
Nucleic Acids Res. 2019 Jan 8;47(D1):D847-D852. doi: 10.1093/nar/gky842.
Ribosome profiling (Ribo-Seq) is a technique that allows for the isolation and sequencing of mRNA fragments protected from nuclease digestion by actively translating ribosomes. Mapping these ribosome footprints to a genome or transcriptome generates quantitative information on translated regions. To provide access to publicly available ribosome profiling data in the context of transcriptomes we developed Trips-Viz (transcriptome-wide information on protein synthesis-visualized). Trips-Viz provides a large range of graphical tools for exploring global properties of translatomes and of individual transcripts. It enables analysis of aligned footprints to evaluate datasets quality, differential gene expression detection, visual identification of upstream ORFs and alternative proteoforms. Trips-Viz is available at https://trips.ucc.ie.
核糖体图谱（Ribo-Seq）是一种技术，可通过保护正在翻译的核糖体免受核酸酶消化来分离和测序 mRNA 片段。将这些核糖体足迹映射到基因组或转录组上，可以生成有关翻译区域的定量信息。为了在转录组的背景下提供对公开可用的核糖体图谱数据的访问，我们开发了 Trips-Viz（蛋白质合成的转录组范围信息可视化）。Trips-Viz 提供了大量图形工具，可用于探索翻译组和单个转录本的全局属性。它可以分析对齐的足迹，以评估数据集的质量，检测差异基因表达，可视化鉴定上游 ORF 和替代蛋白形式。Trips-Viz 可在 https://trips.ucc.ie 上获得。
